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EC number: 202-429-0 | CAS number: 95-53-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (no necropsy and no histopathological examinations were performed)
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
- Reference Type:
- publication
- Title:
- o-TOLUIDINE CAS No: 95-53-4 SIDS Initial Assessment Report.
- Author:
- OECD SIDS
- Year:
- 2 006
- Bibliographic source:
- UNEP Publications
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- Analytical purity not reported. Females were not tested. Fasting of animals not reported. No necropsy and no histopathological examinations. Body weights not recoreded. Few informations on environmental conditions.
- GLP compliance:
- no
- Remarks:
- GLP was not mandatory at the time of the study
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- o-toluidine
- EC Number:
- 202-429-0
- EC Name:
- o-toluidine
- Cas Number:
- 95-53-4
- Molecular formula:
- C7H9N
- IUPAC Name:
- 2-methylaniline
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): o-toluidine
- Analytical purity: no data
- Physical state: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Wistar-II
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Germany
- Age: 6 - 7 weeks
- Weight at study initiation: 160 -180 g
-Housing conditions. animals were housed in groups of 5 per cage
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- single oral application of different doses of undiluted substance to groups of male rats
- Doses:
- 0.600 - 0.650 - 0.700 - 0.800 - 0.900 mL/kg bw, undiluted (corresponding to 600, 650, 700, 800, 900 mg/kg bw (density: 0.998 g/cm³)
- No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs - Statistics:
- Calculation of the median lethal dose (LD50) according to Fink and Hund (Arzneim. Forsch. 15, 1965, 624)
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 750 mg/kg bw
- 95% CL:
- 710 - 790
- Mortality:
- Dose [ml/kg bw]: 600 mg/kg: 0/10 650 mg/kg: 1/10 4d post application 700 mg/kg: 4/10 within 3-4 days post application 800 mg/kg: 6/10 within 2-4 days post application 900 mg/kg: 10/10 within 2-5 days post application
- Clinical signs:
- other: 600 mg/kg: reduced general condition at day 2; 650 mg/kg: reduced general condition, anesthesia day 1 up to day 14; 700 mg/kg: reduced general condition, anaesthesia, increased diurese - day 1 up to day 14; 800 mg/kg: reduced general
Any other information on results incl. tables
MORTALITY:
Dose [ml/kg bw]:
600 mg/kg: 0/10
650 mg/kg: 1/10 4d post application
700 mg/kg: 4/10 within 3-4 days post application
800 mg/kg: 6/10 within 2-4 days post application
900 mg/kg: 10/10 within 2-5 days post application
CLINICAL SIGNS:
600 mg/kg: reduced general condition at day 2
650 mg/kg: reduced general condition, anesthesia
day 1 up to day 14
700 mg/kg: reduced general condition, anaesthesia,
increased diurese - day 1 up to day 14
800 mg/kg: reduced general condition, anaesthesia,
cyanosis, bloody eyes - day 1 up to day 14
900 mg/kg: reduced general condition, anaesthesia,
cyanosis, bloody eyes - day 1 up to time of
death
Applicant's summary and conclusion
- Executive summary:
Löser E (1978)
In male Wistar rats (n = 10) dosed with 0.6, 0.65, 0.7, 0.8 or 0.9 ml/kg bw (app. 600, 650, 700, 800 or 900 mg/kg bw) undiluted o-toluidine, deaths occurred at dose levels of = 0.65 ml/kg bw within 2 - 5 days after application. In moribund animals anesthesia, increased diurese, cyanosis, bloody eyes were observed. No necropsy and no histopathological examinations were performed. From this study a LD50 value of 0.75 ml/kg bw (corresponding to 750 mg/kg bw) was derived. This study was conducted with a method similar to OECD guideline 401 with acceptable deviations (Analytical purity not reported. Females were not tested. Fasting of animals not reported. No necropsy and no histopathological examinations. Body weights not recoreded. Few informations on environmental conditions of the animals.)
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