Registration Dossier
Registration Dossier
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EC number: 202-429-0 | CAS number: 95-53-4
Endpoint summary
Administrative data
Description of key information
Additional information
Concerning the aquatic effects short term toxicity, tests for each trophic level are available and concerning long term toxicity, two tests with species representing two trophic level are available.
The most sensitive species was Daphnia magna followed by algae.
It is not clear whether analytical monitoring was performed in all available test, but studies with analytical monitoring indicated that the substance was stable in water, constant exposure concentrations over the test duration can be assumed.
For various fish species short term toxicity tests are available, with LC50 (96h) values ranging from 82.5 to 151 mg/l.
The most sensitive value on toxicity to fish was determined in a test conducted with a method which was in most parts equivalent to the OECD guideline 204 (Fish, prolonged toxicity test). A 14day-LC50 of 81.3 mg/L was determined on Poecilia reticulata.
Concerning acute toxicity of o-toluidine to the invertebrate Daphnia magna, Maas-Dieperveen reported a 48 hours EC50 value of 0.52 mg/L which is the most sensitive value. The test was performed according to the Dutch Standardization Organization Method NEN 6501 without analytical monitoring.
Concerning the algal toxicity, tests on three different species are available. The lowest available EC50 based on growth rate, was 110.5 mg/l after 72 hours, in a test carried out according to the OECD TG 201 towards Selenastrum capricornutum. A NOEC of 2.91mg/l was determined for the endpoint growth rate.
The lowest chronic test result obtained for Daphnia magna was in a GLP-test according to the OECD TG 202. An NOEC of 0.0126 mg/l and a LOEC of 0.04 mg/l were determined for the endpoint reproduction in a GLP-test according to the OECD TG 202 (CERI, 1996).
Toxicity to Microorganisms was investigated towards Tetrahymena pyriformis.
The toxicity of o-toluidine to Tetrahymena pyriformis was tested in a 40 hours test using the population growth impairment as endpoint. The test was performed according to the method described by Schultz (1997). An EC50 of ca. 155 mg/l was observed (Schultz, 1999).
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