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EC number: 500-012-0 | CAS number: 9004-77-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well reported study for which original report available. Only four strains of bacteria tested. Not to GLP.
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1983 version
- Deviations:
- yes
- Remarks:
- , Only four strains of bacteria tested.
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-(2-(2-butoxyethoxy)ethoxy)ethanol
- EC Number:
- 205-592-6
- EC Name:
- 2-(2-(2-butoxyethoxy)ethoxy)ethanol
- Cas Number:
- 143-22-6
- Molecular formula:
- C10H22O4
- IUPAC Name:
- 2-(2-(2-Butoxyethoxy)ethoxy)ethanol
- Details on test material:
- Test material contained 87.2% of 2-(2-(2-butoxyethoxy)ethoxy)ethanol. Remainder not stated but likely to be higher glycol ethers.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- - Periodically checked for karyotype stability: yes. The Salmonella stains were periodically checked for deep rough character (rfa), UV sensitivity (uvrB), and ampicillin resistance (R factor plasmid).
- Periodically "cleansed" against high spontaneous background: Histidine auxotrophy was automatically checked in each experiment via the spontaneous mutation rate.
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix from Aroclor 1254 induced male SD rats.
- Test concentrations with justification for top dose:
- 20-50000ug per plate
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- Positive controls:
- yes
- Remarks:
- Dissolved in DMSO
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- Migrated to IUCLID6: 5 ug for strains TA100 and TA1535 in the absence of S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- Positive controls:
- yes
- Remarks:
- Dissolved in DMSO
- Positive control substance:
- other: 10ug of 4-nitro-o-phenylenediamine for TA98 in the absence of S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- Positive controls:
- yes
- Remarks:
- Dissolved in DMSO
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Migrated to IUCLID6: as the chloride monohydrate at 100ug for strain TA1537 in the absence of S9.
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- Positive controls:
- yes
- Remarks:
- Dissolved in DMSO
- Positive control substance:
- other: 2-amino anthracene at 10ug/plate for all strains with S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION; in agar (plate incorporation) and preincubation method used.
DURATION
- Preincubation period: 20 mins
- Exposure duration: continuous
- Expression time (cells in growth medium): 48 hours
NUMBER OF REPLICATIONS: 3 - Evaluation criteria:
- Evaluation Criteria: The test material was considered a mutagen if both the mean number of revertant colonies was at least 2 times higher than the
mean of the negative (solvent) control and it induced a reproducible doseresponse relationship over several concentrations. If the dose-response
was not definitive, it was considered to be a presumptive mutagen. If the reversion rates were between 2 and 3 times that of negative controls, the
results were considered equivocal or inconclusive.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- Toxicity : No bacteriotoxic effect was observed.
Mutagenicity: An increase in the number of his+ revertants was not observed both in the standard plate test and in the preincubation test either without S-9 mix or after the addition of a metabolizing system. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'. Remarks: Results for standard test
Any other information on results incl. tables
Standard test: The average number of revertant in the controls for strains TA98, TA100, TA1535, and TA1537 in the absence of S-9 were 23, 114, 16, and 9 respectively. Addition of S-9 to strain TA98 increased the control mutation frequency to 34. S-9 had no effect on the frequency of mutations in the other strains. Positive controls induced an average of from 152 revertants in TA1535 to 1690 revertants in TA100. The number of revertants induced by test material was not increased from that of control at any concentration. The average number of revertants in cultures treated with test material (in the absence or presence of S-9) ranged from 109 to 140 in TA100, 12 to 21 in TA1535, and 8 to11 in TA1537. Similar to control TA98 cultures, the average number of revertants in TA98 cultures treated with test material in the presence of S-9 (33 to 36) were higher than in the absence of S-9 (19 to 24).
Preincubation test: The average number of revertant in the controls for strains TA98, TA100, TA1535, and TA1537 in the absence of S-9 were 24, 111, 17, and 8. Addition of S-9 to strains TA98 and TA1535 increased the control mutation frequency to 33 and 23, respectively. S-9 had no substantial effect on the frequency of mutations in the other strains. Positive controls induced an average of from 94 revertants in TA1537 to 1127 revertants in TA100. The number of revertants induced by test material was not increased from that of control at any concentration. The average number of revertants in cultures treated with test material (in the absence or presence of S-9) ranged from 108 to 135 in TA100 and 7 to11 in TA1537. Similar to control TA98 cultures, the average number of revertants in TA98 and TA1535 cultures treated with test material in the presence of S-9 (35 to 41 and 18 to 26, respectively) were higher than in the absence of S-9 (19 to 24 and 14 to18, respectively).
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
The test substance does not cause reverse gene mutation in bacterial strains. - Executive summary:
In a guideline bacterial gene mutation study (Ames), 2-(2-(2-butoxyethoxy)ethoxy)ethanol (87% pure) did not cause an increase in mutations when tested at up to 5mg/plate with and without S9 metabolic activation using both a standard plate incorporation and pre-incubation methods of application.
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