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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Study meets basic scientific principles of an acute toxicity study reporting on mortalities and clinical signs of intoxication occurring within 24 h post application, no information on purity of the test substance, sufficient documentation of test results to be accepted as key study
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1948
Report Date:
1948

Materials and methods

Principles of method if other than guideline:
Observation of mortalities and clinical signs of intoxication occuring within 24 h after i.p. application of two different grades of HQ
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Hydroquinone TEC pure (food grade), Hydroquinone photographic (photo grade)
- Analytical purity: no data
- Batch: no data

Test animals

Species:
rat
Strain:
other: albino
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Doses:
78 - 300 mg/kg bw as 1% solution
No. of animals per sex per dose:
5 for each grade of HQ
Details on study design:
- Duration of observation period following administration: 24 h
- Other examinations performed: clinical signs

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LD50
Effect level:
160 mg/kg bw
Based on:
test mat.
Remarks on result:
other: HQ food grade
Sex:
male/female
Dose descriptor:
LD50
Effect level:
175 mg/kg bw
Based on:
test mat.
Remarks on result:
other: HQ photo grade
Mortality:
HQ TEC pure: 0% up to 135 mg/kg; 40% at 135 mg/kg (2nd experiment); 40% at 154 mg/kg; 100% at 202 mg/kg; 80% at 225 mg/kg; 60% at 230 and 300 mg/kg; deaths occurred also several hrs p.a.
HQ photo grade: 0% at 81 mg/kg; 40% at 114 and 164 mg/kg; 60% at 210 mg/kg; 100% at 225 mg/kg; 80% at 229 mg/kg; 60% at 263 mg/kg; 80% at 266 and 269 mg/kg; deaths occurred witin 10 to 30 min p.a.
Clinical signs:
Clonic convulsive movements proceeding into running movements; heart observed to beat for several minutes after respiration ceased

Applicant's summary and conclusion

Conclusions:
After intraperitoneal injection LD50 values in the range of 160 or 175 mg/kg bw were found in male or female Albino rats.
Executive summary:

Mortalities and clinical signs of intoxication were observed in male and female albino rats after i.p.application of two different grades of HQ (food grade and photographic grade) for a total observation period of 24 hrs. In male and female rats, LD50 values were in the range of 160 or 175 mg/kg bw for the food grade and photographic grade, respectively. These values were found to be similar to intraperitoneal LD50 values of other species, as e.g. mice, rabbits, and guinea pigs. Clinical signs were characterized by clonic convulsive movements proceeding into running movements pointing to central nervous system effects.