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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)
Version / remarks:
Cited as Directive 84/449/EEC, C.3
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): municipal STP, Marl-Ost
- Water filtered: yes
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
11.6 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: 2 mL mineral nutrient solution, 2 mL micronutrients solution, 2 mL vitamin solution, 1 mL inoculum, test item solution resulting in approx. 10 mg DOC/L, water added up to a volume of 2 L
- Test temperature: 25-25.1°C
- pH adjusted: no data
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Number of inoculum blank samples: 2
- Number of reference substance samples: 2
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
11
Sampling time:
28 d
Details on results:
Kinetic of test substance (in % DOC reduction):
7% after 7 day(s)
11% after 14 day(s)
4% after 21 day(s)
7% after 27 day(s)
11% after 28 day(s)

BOD5 / COD results

Results with reference substance:
Kinetic of control substance (in % DOC reduction):
98% after 14 day(s)
100% after 28 day(s)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not readily biodegradable
Conclusions:
In this screening test for ready degradability the test item triacetonamine showed a degradation of 11% after 28 days.