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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP, Guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
Directive 92/69/EEC
Deviations:
no
Principles of method if other than guideline:
Two tests with different test concentrations were conducted.
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
The test concentrations were checked analytically by HPLC at test start and every 24 hours.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solutions were prepared separately. The stock solutions were prepared freshly every day.
Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: Feathead minnow
- Source: BIO INTERNATIONAL B.V., Roermond, Netherlands
- Length at study initiation: 3.6 - 4.6 cm (test 1), 4.6 - 5.8 cm (test 2)
- Weight at study initiation: 0.45 - 0.96 g (test 1), 0.67 - 1.62 g (test 2)
- Feeding during test: No feeding occurred during the test as well as 24 h before test start.

ACCLIMATION
- Acclimation conditions: fish were acclimatized in water of the same quality as used in the test.
- Food type: They were fed daily with TetraMin Hauptfutter (Tetra Werke, Melle, Germany).
- Feeding frequency: daily
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no
Hardness:
no data
Test temperature:
23 ± 1 °C
pH:
8.2 - 8.8 (test 1)
7.6 - 8.4 (test 2)
Dissolved oxygen:
7.4 - 8.4 mg/L (test 1)
6.3 - 8.5 mg/L (test 2)
Salinity:
not applicable
Nominal and measured concentrations:
Nominal test concentrations mg/L (test 1):
control, 0.34, 0.75, 0.80, 1.00, 1.20, 1.65, 3.64, 8.00

Nominal test concentrations mg/L (test 2):
control, 0.80, 1.00, 1.20

Mean measured test concentrations mg/L (test 1):
control, 0.17, 0.52, 1.18, 2.58, 4.50
Mean measured test concentrations mg/L (test 2):
0.77, 0.82, 1.36
Details on test conditions:
TEST SYSTEM
- Test vessel: 15-L glass aquaria
- Fill volume: 10 L
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): 2.5 L/h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.7 mg/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified drinking water

OTHER TEST CONDITIONS
- Photoperiod: 14h light/10h dark.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality after 0, 24, 48, 72 and 96 hours
Reference substance (positive control):
not required
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
0.17 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.61 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
96 h
Dose descriptor:
LC10
Effect conc.:
0.41 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
0.52 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The measured concentrations deviated by more than 20 % from nominal values at all dose levels of test 1 and 2. Thus, mean measured concentrations were taken as basis for the calculation of the lethal effect concentrations.
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
LC20 and LC50 values were calculated using probit analysis (3)
Sublethal observations / clinical signs:

Cumulative Mortality

24h

48h

72h

96h

0

0

0

0

0

0

0

0

0

1

1

2

0

0

4

5

0

3

7

9

0

9

9

9

0

10

10

10

10

10

10

10

10

10

10

10

Validity criteria fulfilled:
yes
Conclusions:
The 96-h LC50 of Allyl methacrylate in fathead minnow (Pimephales promelas) was 0.61 mg/L. The 96 h LOEC was 0.52 mg/L.
Executive summary:

In a 96-h acute toxicity study (EC dir. 92/69/EEC) fathead minnow (Pimephales promelas) were exposed to Allyl methacrylate with nominal concentrations of 0.34, 0.75, 1.65, 3.64, 8.00 mg/l (test 1) and 0.80, 1.00, 1.20 mg/l (test 2) under flow through conditions. The 96-h LC50 was 0.61 mg/L. The 96 -h LOEC was 0.52 mg/L.

  

Results Synopsis

Test organism size/weight: 3.6 - 5.8 cm; 0,7 - 1.1 g

Test Type: Flow through

LC50: 0.61 mg/L 95% C.I.: 0.53 – 0.71 mg/L

LOEC: 0.52 mg/L

 

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

 

Description of key information

LC50 96 h (flow-through): 0.61 mg/L;
Acute (short term) aquatic hazard, Category Acute 1 according to Regulation (EC) No 1272/2008 (CLP).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.61 mg/L

Additional information

The acute toxicity of ally methacrylate to fish was tested in a study following Directive 92/69/EEC, C.1. Pimephales promelas was exposed to the substance for 96 hours in a flow-through system. An LC50 of 0.61 mg/L based on measured concentrations was determined (Roehm GmbH, 2001). The test result is supported by two further tests on the acute toxicity of the substance to juvenile P. promelas. In a test following American Society for Testing and Materials recommendations, the toxicity to fish was tested in a flow-through system. At test termination after 96 hours a LC50 of 0.99 mg/L was detected (Russom et al. 1988). In a second study P. promelas were exposed in a static test system for 96 hours. An LC50 of 0.99 mg/L was determined (Geiger et al. 1984). Both test results refer to nominal concentrations.