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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-08-09
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N-[3-(dimethylamino)propyl]stearamide
EC Number:
231-609-1
EC Name:
N-[3-(dimethylamino)propyl]stearamide
Cas Number:
7651-02-7
Molecular formula:
C23H48N2O
IUPAC Name:
N-[3-(dimethylamino)propyl]octadecanamide
Test material form:
solid: pellets
Details on test material:
- Name of test material (as cited in study report): Stearylamidopropyl dimethylamine

Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
waxy solid

Sampling and analysis

Analytical monitoring:
no

Test organisms

Details on inoculum:
Activated sludge from Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire
Sludge kept at 21 deg C, buffered to pH 7 with NaOH
3.9 g/L solids

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Hardness:
not given
Test temperature:
21 deg C
pH:
pH = 7
Dissolved oxygen:
saturation
Nominal and measured concentrations:
Range finding test: 1-10-100-1000 mg/L
Definitive test: 100 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 100 - < 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: based on range finding & definitive test
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
no details available
Results with reference substance (positive control):
EC50 (3h) 9.8 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In an OECD 209 Sludge respiration inhibition test no effect was observed at the limit test concentration of 100 mg/L. Based on the range finding test it can be estimated that the EC50 (3h) is between 100 and 1000 mg/L.