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EC number: 428-710-1 | CAS number: 207574-76-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Commission Directive 92/69/EEC, 1992
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: nominal 100 mg/L and control
- Sampling intervals: start (0 h), and at test end (48 h)
- Sampling method:
At test start: unfiltrated and filtrated (maximum pore size approx. 1.2 µm) samples from the freshly prepared undiluted supersaturated suspension.
At test end: the contents of the test beakers were pooled and filtered (maximum pore size approx. 1.2 μm). In addition, undiluted supersaturated suspensions, incubated in parallel without daphnids under the same conditions as in the actual test were collected (collecting of representative suspension-samples from the actual test itself was not possible since the test item was not homogeneously distributed in the test medium).
Control samples were taken directly from the test beakers.
Samples were taken in duplicate.
- Sample storage conditions before analysis: All samples were deep-frozen immediately after sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Due to the low water solubility of the test item the supersaturated suspension with a nominal concentration of 100 mg/I was prepared as follows: 262 mg of the test item were suspended in a small volume of test water by using a homogenisator. Then the mixture was transferred quantitatively into an adequate volume of test water to obtain a final concentration of nominal 100 mg/L. This suspension was treated ultrasonically for 15 minutes. Then, the supersaturated suspension was stirred by a magnetic stirrer at room temperature in the dark over 96 hours to dissolve a maximum concentration of the test item in the suspension. No auxiliary solvent or emulsifier was used. The stirring period of 96 hours was chosen according to the results of a pre-test, which showed that during this time no significant loss of the test item was to be expected. After stirring and after another 15 minutes ultrasonic treatment prior to the start of the test, a homogeneous suspension was obtained. The undiluted supersaturated suspension with the nominal concentration of 100 mg/L dissolved and suspended test item was used as test medium.
- Controls: Test water without addition of the test item
- Evidence of undissolved material (e.g. precipitate, surface film, etc): At the test start, the test substance was very finely and homogeneously suspended in the test medium. After about one hour, test substance particles were observed at the water surface. After one day, substance particles were observed at the water surface and the bottom of the beaker. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flee
- Strain/clone: STRAUSS
- Source: in-house culture
- Feeding during test: none
ACCLIMATION
- Acclimation period: none. Breeding was performed in reconstituted water of identical quality (regarding pH, main ions and total hardness) and under identical temperature and light conditions as in the tests.
- Type and amount of food: not reported
- Health during acclimation (any mortality observed): not reported
The daphnids used for the test were 6 - 24 hours old and were not first brood progeny. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 2.5 mmol/l as CaCO3
- Test temperature:
- 20-22°C
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- >= 8.5 mg/l
- Nominal and measured concentrations:
- nominal loading rate: 100 mg/L
measured test concentrations
- unfiltered sample of the suspensions: 88% and 83% of the nominal value (total mean 85%)
- dissolved and very finely suspended test item (measured in the filtrate of the samples): below the limit of detection (LOD) of approx. 1.0 mg/L.
The biological results are based on the loading rate (nominal concentration) of the test item. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beakers containing 50 mL test medium
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 5 mL/animal
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water acc. to ISO 6341. Analytical grade salts and additives were dissolved in purified water.
- Ca/Mg ratio: 4:1
- Alkalinity: 0.8 mM
- Culture medium different from test medium: no
- Intervals of water quality measurement: water temperature, oxygen concentration and pH were measured at the start and at the end of the test. The appearance of the test media was recorded at test start and after 24 and 48 hours.
OTHER TEST CONDITIONS
- Adjustment of pH: not necessary
- Photoperiod: 16-hour light to 8-hour dark cycle with a 30-minute transition period
- Light intensity: between 200 and 1200 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Daphnids were inspected for immobility, mortality and other signs of intoxication after 24 and 48 hours of exposure.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)
RANGE-FINDING STUDY
- Results used to determine the conditions for the definitive study: the test concentration was based on the results of a range-finding test and the results of pre-experiments to the solubility of the test material. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- other: mobility and sub-lethal effects
- Details on results:
- Behavioural abnormalities:
- Other biological observations: none; no immobility or mortality or other signs of intoxication were observed.
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Effect concentrations exceeding solubility of substance in test medium: Yes, the solubility of the test item in test water was below the LOQ of the analytical method (< 1 mg/L). - Results with reference substance (positive control):
- not reported
- Reported statistics and error estimates:
- not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results indicate that the test material is not toxic to daphnids up to the limits of its water solubility and a NOEC and thus a PNEC cannot be determined.
Since no classification criteria according to GHS/CLP (Regulation (EC) No. 1272/2008) are fulfilled, the substance is not classified for environmental hazards. - Executive summary:
Due to the low water solubilty of the test item, an unfiltrated supersaturated suspension with a nominal concentration of 100 mg/l was used as the test medium, containing a max. concentration of the test substance.
During the test period the total mean measured concentration of dissolved and suspended test substance in the unfiltrated duplicate samples of the suspension was 85% of the nominal value. Under the test conditions, the concentration was sufficiently constant during the 48-hours test period. The concentration of dissolved and very finely suspended test substance was below the detection limit of approx. 1.0 mg/l.
As no moratlity was observed, the 48-hour EL10 (NOELR) and thus a PNEC cannot be determined.
Reference
Description of key information
effect value for Daphnia magna, based on loading rates (OECD 202, EU C.2):
48-h EL50: >100 mg/L (solubility in test water < 1 mg/L)
Key value for chemical safety assessment
Additional information
EL50 for Daphnia magna >100 mg/L
The results indicate that the test material is not toxic to daphnia up to the limits of its water solubility and a NOEC and thus a PNEC cannot be determined.
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