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No short-term terrestrial test data is available for primary fatty amine ethoxylates. One long-term terrestrial test was performed with Bis (2-hydroxyethyl) oleyl amine (CAS No 25307-17-9).

The effects of Bis (2-hydroxyethyl) oleyl amine (CAS No 25307-17-9) on mortality, biomass and reproduction of Eisenia fetida was tested according to OECD 222 under a static exposure for 56 days. Natural soil was used as substrate, and the different concentration of the substance mixed with the substrate. The concentrations applied were 62.5, 125, 250, 500 and 1000 mg/kg soil dry weight. In addition tests using control and vehicle control were performed.

The test is valid without restrictions and the results are reliable. After 28 days of exposure, no effects on survival of the adult worms were observed in all concentrations.  However, at 1000 mg/kg soil dry weight the biomass increase was statistically significantly reduced compared to the control.

After a further four weeks,compared to the control, the reproduction rate was statistically significantly reduced at 1000 mg/kg soil dry weight. All other tested application rates showed no statistically significant reduction compared to the control.  Hence, the NOEC is 500 mg/kg dw soil.