Reaction products of monoethanolamine and boric acid (1:1)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

96 hours

Reliability:

1 (reliable without restriction)

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion in water
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):water
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)):100mg/litre

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Acclimitisation - stock held from 23 February 1990 and acclimitised to test conditions from 27th February to 6th March 1990

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

50 mg/l as CaCO3

Test temperature:

21 +/- 1 deg C

pH:

7 - 7.7

Dissolved oxygen:

>= 8.2 mg O2/l

Salinity:

Not relevant

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume:glass
- Aeration:via narrow bore glass tubes
- Renewal rate of test solution (frequency/flow rate):daily
- No. of organisms per vessel:20
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):1
- Biomass loading rate:0.17 g bodyweight per litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:laboratory tap water, dechlorinated by the addition of sodium thiosulphate

OTHER TEST CONDITIONS

- Photoperiod: 16h light 8 h dark
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS

- Test concentrations:100 mg/l
- Results used to determine the conditions for the definitive study:100 mg/l

Reference substance (positive control):

no

Duration:

3 h

Dose descriptor:

LC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

6 h

Dose descriptor:

LC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities:none
- Other biological observations: none
- Mortality of control:none
- Other adverse effects control:none
- Abnormal responses:none

Results with reference substance (positive control):

none

Reported statistics and error estimates:

There were no mortalities or other adverse reactions to exposure in 20 fish exposed to a test concentration of 100 mg/l for a period of 96 hours.

Sublethal observations / clinical signs:

none

Validity criteria fulfilled:

yes

Remarks:

0% mortality in controls.

Conclusions:

The 96 h LC50 of Reaction products of monoethanolamine and boric acid 1:1 in a GLP OECD 203 study is greater than 100 mg/l (no mortalities or other adverse reactions).

Executive summary:

There were no mortalities or other adverse reactions to exposure in 20 fish exposed to a test concentration of 100 mg/l for a period of 96 hours.

Description of key information

The 96 h LC50 of Reaction products of monoethanolamine and boric acid 1:1 in a GLP OECD 203 study is greater than 100 mg/l (no mortalities or other adverse reactions).