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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
96 hours
Reliability:
1 (reliable without restriction)
Reason / purpose:
reference to other study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion in water
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):water
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)):100mg/litre
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Acclimatisation - stock held from 23 February 1990 and acclimatised to test conditions from 27th February to 6th March 1990
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
None
Hardness:
50 mg/l as CaCO3
Test temperature:
21 +/- 1 deg C
pH:
7 - 7.7
Dissolved oxygen:
>= 8.2 mg O2/l
Salinity:
Not relevant
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume:glass
- Aeration:via narrow bore glass tubes
- Renewal rate of test solution (frequency/flow rate):daily
- No. of organisms per vessel:20
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):1
- Biomass loading rate:0.17 g bodyweight per litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:laboratory tap water, dechlorinated by the addition of sodium thiosulphate

OTHER TEST CONDITIONS

- Photoperiod: 16h light 8 h dark
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS

- Test concentrations:100 mg/l
- Results used to determine the conditions for the definitive study:100 mg/l
Reference substance (positive control):
no
Duration:
3 h
Dose descriptor:
LC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
6 h
Dose descriptor:
LC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities:none
- Other biological observations: none
- Mortality of control:none
- Other adverse effects control:none
- Abnormal responses:none
Results with reference substance (positive control):
none
Reported statistics and error estimates:
There were no mortalities or other adverse reactions to exposure in 20 fish exposed to a test concentration of 100 mg/l for a period of 96 hours.

none

Validity criteria fulfilled:
yes
Remarks:
0% mortality in controls.
Conclusions:
The 96 h LC50 of Reaction products of monoethanolamine and boric acid 1:1 in a GLP OECD 203 study is greater than 100 mg/l (no mortalities or other adverse reactions).
Executive summary:

There were no mortalities or other adverse reactions to exposure in 20 fish exposed to a test concentration of 100 mg/l for a period of 96 hours.

Description of key information

The 96 h LC50 of Reaction products of monoethanolamine and boric acid 1:1 in a GLP OECD 203 study is greater than 100 mg/l (no mortalities or other adverse reactions).

Key value for chemical safety assessment

Additional information