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Short-term toxicity to aquatic invertebrates

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Description of key information

EC50 aquatic invertebrates: 0.72 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.72 mg/L

Additional information

In the GLP compliant Klimisch 1 study from Ágh (2012), the acute toxicity of Camphene, technical grade, solid to Daphnia magna was determined according to OECD 202 and EU method C.2 in a semi-static test (24 hour renewal interval) . The test item has a purity of 82.6 % by weight and contains 12.4 % Tricyclene as impurity. A supersaturated solution (nominal loading: 1000 mg/L) was first prepared by mixing an excess of the test item in dilution water (Elendt M4 medium). This mixture was shaken overnight and then the non-dissolved test material was separated to give the stock solution at the limit of solubility level in the test medium. This stock solution was further diluted with dilution water to result in following dilutions:26.0, 36.4, 51.0, 71.4 and 100 % v/v saturated solution. Due to immobilization at 24 hours in the lowest test concentration, further dilutions with 13.3 and 18.6% were set up (experiment 2). The concentration of Camphene was experimentally determined. and following geometric mean measured concentrations were obtained: Experiment I: 0.83, 1.12, 1.35, 2.10 and 3.29 mg/L and Experiment II: 0.48 and 0.73 mg/L. After 48 hours, 0,0, 55, 80, 100, 100, 100 and 100% immobilisation was observed in the control and at mean measured 0.48, 0.73, 0.83, 1.12, 1.35, 2.10 and 3.29 mg/L. During the 24 hour aging period of the solutions the concentration of the test item declined to about 1/3 of the initial concentrations. Therefore, the concentrations were based on mean measured concentrations. The NOEC was 0.48 mg/L, the EC50 was calculated to be 0.72 mg/L.

The results of this study are considered relevant and reliable for the risk assessment.

In the Klimisch 3 study from LeBlanc (1980) the acute toxicity of camphene was determined on Daphnia magna using a static test design. The daphnia were exposed to several concentrations of test substance for 48 hours at 22±1 ºC. Test method according to US EPA (1975): Methods for acute toxicity tests with fish, macro invertebrates and amphibiens. After 48 hours, the EC50 was 22 (13 - 36) mg/L (basis for effect: mortality). Since no dose verification was performed, the test is was classified as Klimisch 3 study. The results from this study will not be taken for the risk assessment.