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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
02 Sep 1987 - 22 Sep 1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Principles of method if other than guideline:
Group of rats were exposed to test substance vapour for four hours and LC50 was determined.
GLP compliance:
not specified
Test type:
fixed concentration procedure
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Hydrocarbons, C6-C7, n-alkanes, isoalkanes, cyclics, <5% n-hexane
EC Number:
921-024-6
Molecular formula:
none available - not a single isomer - see remarks
IUPAC Name:
Hydrocarbons, C6-C7, n-alkanes, isoalkanes, cyclics, <5% n-hexane
Details on test material:
- Name of test material (as cited in study report): SBP 60/95 LNH
- Physical state: liquid
- Analytical purity: 100% pure commercial product
- Storage condition of test material: at room temperature
- Other: colourless

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Interfauna UK Limited
- Age at study initiation: 6-8 weeks
- Weight at study initiation: 200 g
- Housing: polypropylene cages (38x56x18 cm)
- Diet: free access to a measured excess amount of food
- Water (ad libitum): tap water



ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21±0.8 - 25±1.3
- Humidity (%): 61±6.6

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: vapour generator plus exposure chamber
- Exposure chamber volume: 120 L
- Method of holding animals in test chamber: wire mesh compartments
- Source and rate of air: supply of clean dried air was connected to the generator and the supply pressure was adjusted to give a flow rate of 25 L per minute
- Temperature in air chamber: 25±0.33-25.9 °C


TEST ATMOSPHERE
- Brief description of analytical method used: analysed with a gas chromatograph. Samples were drawn through a gas absorption trap, containing 20 ml of acetone and cooled to -70 °C. Sampling rate was 2 litres/minute and the volume of air sample was measured with a wet-type gas meter.
- Samples taken from breathing zone: yes


Analytical verification of test atmosphere concentrations:
yes
Remarks:
Pye Unicam PU 4550 fitted with a flame ionisation detector. Pye Unicam PU 4700 autosampler.
Duration of exposure:
ca. 4 h
Concentrations:
25.2 mg/L air
No. of animals per sex per dose:
5
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical signs at least twice daily, body weight daily, food and water consumption daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 25.2 mg/L air
Exp. duration:
4 h
Mortality:
There were no deaths during the study.
Clinical signs:
other: restless behavior and increase in respiration rate during the first 15 minutes; partial closing of the eyes
Body weight:
no effects
Gross pathology:
The lung weight to bodyweight ratio for all rats was considered to be within normal limits. No other macroscopic abnormalities were observed.
Other findings:
Food consumption was slightly reduced for 1 day following exposure. Water consumption was not affected by exposure.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Remarks:
Migrated information
Conclusions:
Under the conditions of the study a 4 hour LC50 >25.2 mg/L air was determined for the test substance, hydrocarbons, C6-C7, n-alkanes, Isoalkanes, cyclics < 5% hexane.
Executive summary:

Under the conditions of the study a 4 hour LC50 >25.2 mg/L air was determined for the test substance, hydrocarbons, C6-C7, n-alkanes, Isoalkanes, cyclics, < 5% n-hexane.