Registration Dossier

Administrative data

Endpoint:
basic toxicokinetics
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Study period:
2011
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Method not validated

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011

Materials and methods

Objective of study:
metabolism
Principles of method if other than guideline:
Aqueous solutions of the test material were analysed via gas chromatography–mass spectrometry (GC-MS) in order to detect cleavage products. Fragments bearing CF-3 groups at m/z 69 should be identified. The results obtained with either neutral or acidic solutions (water or hydrochloric acid solutions with pH values of 7 or 2, respectively) were compared.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
no

Results and discussion

Main ADME results
Type:
metabolism
Results:
no cleavage products identified under the conditions used in GC-MS analysis

Metabolite characterisation studies

Metabolites identified:
no
Details on metabolites:
Results
m/z 69 was used for SIM-mode analysis. The following table shows the differences which were found for the two solvents (see table 1 in "Any other information on results incl. tables").
The signals at 25.51 min, 26.75 min and 28.25 min are close to the noise. The full-MS mode (m/z 35-650) shows that all peaks for which a difference could be found are relatively small (≤10%).
Some differences in the chromatograms were found (s. table). However, it was not possible to clearly identify cleavage products of the test material under the used conditions.

Any other information on results incl. tables

Table 1: Peaks in H2O and HCl solution   Retention time [min] Difference in relative intensity 
 HCl  24.73  -
   25.51 -
  26.75   -
     28.25  -
    29.39  -
   48.90  yes
       
 H2O  not existing  -
    not existing   -
     not existing  -
     not existing  -
     not existing  -
     48.90  yes

Applicant's summary and conclusion

Conclusions:
Scope of this project was to find cleavage products of the test item. Hydrochloric acid was used for hydrolysis (in order to simulate the acidic conditions of the stomach) and GC-MS was used to detect fragments bearing CF3-groups at m/z 69. An aqueous solution of the test item was prepared as reference. Some differences in the chromatograms were found (s. table). In this study no fragments containing CF3-groups could be identified leading to the conclusion that no cleavage of the pigment occurs under the acid conditions applied.