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Acute aquatic toxicity studies are available for three trophic levels.

The most sensitive organism in acute toxicity testing was Scenedesmus subspicatus with an EC50 of 2.4 mg/l based on growth rate. For fish (Leuciscus idus) a LC50 of 14.66 mg/l was determined and in Daphnia magna the EC50 was 17.48 mg/l.

Chronic aquatic toxicity studies are not available. Considering the a/c-ratio, corresponding studies are not considered to have a relevant influence on the risk assessment and thus no further studies are proposed.

The PNEC calculations are based on the results of the acute toxicity test performed with Sc. subspicatus. For the sediment and terrestric compartments the equilibrium partitioning method has been used.

The PNEC STP was calculated using the EC10 of 580 mg/l determined in a respiration inhibition test with the single species Ps. putida.