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EC number: 201-116-6 | CAS number: 78-42-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-12-24 to 1998-07-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was conducted in accordance with international guidelines and in accordance with GLP. All relevant validity criteria were met.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: Control, aids control ( 50 mg/L HCO-40), 1.0, 3.2 and 10.0 mg/L. For all concentrations (1 test vessel per concentration), 0.75 mL of test solution was collected four times during the exposure period before and after water renewal, and analysed for test substance concntration.
- Vehicle:
- yes
- Details on test solutions:
- 100 mg of test substance was dissolved in 250 mg of DMF, 250 mg HCO-40 was added and the solution was mixed. The solution was diluted in 100 mL od of pure water to make a stock of 1000 mg/L. A stock of solvent controls was also made at 5000 mg/L (DMF:HCO-40, 50:50) without test substance. The appropriate amount of stock was added to a 1 L volumetric flask with 1 L Elendt M4 medium to acheive each test concntration. 80 mL of the corresponding solution was used per replicate test vessel (n =10) at each test concentration or control.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Source:National Institute for Environmental Studies, Japan (18 July 1995) and continuously reared at Yokohama Laboratory since obatiainment in Elendt M4 medium.
- Stocks were created with healthy females, the juvenilles from these females were sperated and further cultured until maturation. The neonates (not first brood) from the final subculture were used in the test.
- Test Daphnia were < 24 hours old
- Food: Chlorella vulgaris, 0.15 mg C (organic carbon)/ day
- Exposure method: semi-static (3 times per week, replace the total amount of the test solution) - Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
- Post exposure observation period:
- Dissolved oxygen (DO), pH, water temperature and water hardness was measured before and after water renewals.
Parental dphnid mortality and mobility was checked daily. Number of juviniles, dead juveniles, aborted and dormant eggs were counted daily, and removed after counts. Days to first brood was also recorded. - Hardness:
- 235 - 305 mg CaCO3/L
- Test temperature:
- 20.0 - 20.9 °C
- pH:
- pH 7.2 - 8.0
- Dissolved oxygen:
- 6.2 -8.8 mg O2/L
- Nominal and measured concentrations:
- Nominal concentrations: control (0), vehicle control (50 mg/L of vehicle only), 1.00, 3.20, 10.0 mg/L. Measured concentrations were 82-104 %, within 80-120 % as recommended by the guideline. Nominal concentrations were used to estimate and determine the endpoints within the report.
- Details on test conditions:
- Daphnia were seeded at a density of 1 daphnid/test vessel(80mL) with 10 replicate dpahnids per concnetration. Daphnids were fed Chlorella vulgaris at 0.15 mg C/daphnid/day. Elendt M4 medium was used as the holding water and test medium. The temperature was 20 ± 1 ℃, with a photoperiod of 16 hours light (1200 lux) / 8 hours dark, pH was between 6-9 and dissolved oxygen was 6-9 mg O2/L. The medium of each test vessel/ control was renewed 3 times per week .
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichormate
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Nominal values were adopted for calculation of effect concentration, because test substance concentrations were within ± 20 % of nominal values when they were measured at the time of preparation and before renewal of test solution during exposure period.
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Nominal values were adopted for calculation of effect concentration, because test substance concentrations were within ± 20 % of nominal values when they were measured at the time of preparation and before renewal of test solution during exposure period.
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Nominal values were adopted for calculation of effect concentration, because test substance concentrations were within ± 20 % of nominal values when they were measured at the time of preparation and before renewal of test solution during exposure period.
- Details on results:
- measured concntrations were 83 - 104 % nominal. Validity criteria were met.
- > 60 live offspring produced per parent female in the control groups
- < 20 % mortality during the test.
Medium parameters remained optimal for Daphnia magna health. - Results with reference substance (positive control):
- 48 hour EC50 = 0.66 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 21 day LC50 was estimated to be > 10 mg/L. The NOEC and LOEC for reproduction were 1 mg/L and 3.2 ,g/L, repsectively.
- Executive summary:
The toxicity of tris(2 -ethylhexyl)phosphate to Daphnia magna was studied during a Daphnia magna 21 day reproduction test (according to OECD guideline 211; Daphnia manga Reproduction Test). Test solutions were made in Elendt M4 medium, concntrations were 0, 0 (with solvent control), 1, 3.2 and 10 mg/L. A dispersant was used to enhance the solubility of the test item in the test medium. The NOEC and LOEC were determined using the Dunnett's test.
The LC50 was estimated to be > 10 mg/L; and the reproductive NOEC and LOEC were determined to be 1 mg/L and 3.20 mg/L, respectively.
Reference
Table 1: Measured concentration as % nominal concntration.
|
|
Measured concentration |
||||||||
Nominal Concentration |
DAY |
0 |
2 |
7 |
9 |
9 |
12 |
19 |
21 |
|
Control |
|
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
|
Solvent control |
|
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
|
1.00 |
|
83 |
83 |
88 |
87 |
96 |
89 |
96 |
85 |
|
3.20 |
|
85 |
82 |
95 |
87 |
100 |
83 |
91 |
86 |
|
10.0 |
|
96 |
84 |
94 |
92 |
102 |
95 |
104 |
104 |
Cumulative Numbers of Juveniles Produced per Adult Alive for 21 Days in Each Test Vessels and Results of Statistical Comparison of the Mean Values (Dunnett’s Multicomparison Test)
Nominal concentration mg/L | |||||
Vessel no. | Control | Solvent control | 1.00 | 3.20 | 10.0 |
1 | 130 | D | 167 | 134 | D |
2 | 142 | 166 | 172 | 135 | 98 |
3 | 142 | 160 | 149 | 138 | D |
4 | 125 | 168 | 144 | 121 | 101 |
5 | 148 | 163 | 126 | 148 | 84 |
6 | 137 | 132 | 142 | 133 | 125 |
7 | 145 | 155 | 159 | 154 | 115 |
8 | 140 | 138 | 166 | 138 | 137 |
9 | 160 | 160 | 148 | 117 | 102 |
10 | 150 | 140 | 163 | 140 | 108 |
MeanS.D. | 141.910.0 | 153.613.4 | 153.614.3 | 135.811.0 | 108.816.6 |
Inhibition rate %Significant difference | 0.0- | 11.6* | 29.2** |
D: Were not included for calculatioa because the parentalDaphniawas dead during a 21 -days testing period
-: Indicates no si gnificant difference
*: Indicates a significant difference (α = 0.05) from the solvent control
**: Indicates a significant difference (α = 0.01) from the solvent control
No Observed Effect Concentration (NOEC): 1.00 mg/L
Lowest Observed Effect Concentration (LOEC): 3.20 mg/L
Description of key information
NOEC, LOEC and EC50 (21 d Daphnia magna reproduction test); 1 mg/L, 3.2 mg/L and >10 mg/L; OECD 211, Anon (1998)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1 mg/L
Additional information
The toxicity of the substance to Daphnia magna during a 21 d reproduction was assessed in line with OECD 211 guidelines (Anon, 1998). The NOEC was determined to be 1 mg/L, derived from a significant reduction in fecundity at 21 days. A dispersant was used to enhance the solubility of the test item in test medium and the resulting NOEC is orders of magnitude higher than the limit of water solubility.
Due to the substance not causing reproductive effects at, or above, the limit of water solubility the substance was concluded as having no long-term toxicity to aquatic invertebrates and no hazard has been identified for this endpoint in accordance with REACH Annex I (section 3).
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