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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From September 08, 1993 to September 21, 1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Test temperature:
20-22°C
pH:
7.1
Nominal and measured concentrations:
0.24 - 31.3 mg/L nominal (equivalent to 0.07 - 9.39 mg a.i./L)
Reference substance (positive control):
yes
Remarks:
2,4-dichlorophenol
Key result
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
ca. 1.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: equivalent to 0.48 mg a.i./L
Key result
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
ca. 3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: equivalent to 0.96 mg a.i./L
Key result
Duration:
16 h
Dose descriptor:
EC100
Effect conc.:
ca. 2.31 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: equivalent to 2.31 mg a.i./L
Validity criteria fulfilled:
yes
Conclusions:
Under the study conditions, the EC10, EC50 and EC100 for P. putida were 1.6, 3.2 and 7.7 mg/L (nominal), respectively (equivalent to 0.48, 0.96 and 2.31 mg a.i./L, respectively).
Executive summary:

A study was conducted to determine the toxicity of the test substance, C16 TMAC (30% active in water), to the microorganism according to DIN Norm 38 412 Part 8, in compliance with GLP. In this study, Pseudomonas putida were exposed to the test substance at nominal concentrations of 0.24 - 31.3 mg/L (i.e., 0.07 - 9.39 mg a.i./L) for 16 h. A positive control, 2,4 dichlorophenol, was also included in the study. No analytical verification of the test concentration was performed. Under the study conditions, the EC10, EC50 and EC100 for P. putida were determined to be 1.6, 3.2 and 7.7 mg/L (nominal), respectively (equivalent to 0.48, 0.96 and 2.31 mg a.i./L, respectively) (Stuhlfauth, 1993).

Description of key information

The test substance is considered toxic to P. putida with a 16 h EC50 and EC10 values at 0.96 and 0.4 mg a.i./L (nominal) respectively.

Key value for chemical safety assessment

EC50 for microorganisms:
0.96 mg/L
EC10 or NOEC for microorganisms:
0.4 mg/L

Additional information

A study was conducted to determine the toxicity of the test substance, C16 TMAC (30% active in water), to the microorganism according to DIN Norm 38 412 Part 8, in compliance with GLP. In this study,Pseudomonas putida were exposed to the test substance at nominal concentrations of 0.24 - 31.3 mg/L (i.e., 0.07 - 9.14 mg a.i./L) for 16 hours. A positive control, 2,4 dichlorophenol, was also included in the study. No analytical verification of the test concentration was performed. Under the test conditions, the EC10, EC50 and EC100 values for P. putida were determined to be 1.6, 3.2 and 7.7 mg/L (nominal), equivalent to 0.40, 0.96 and 2.31 mg a.i./L, respectively (Stuhlfauth, 1993).