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EC number: 224-235-5 | CAS number: 4259-15-8
Ecotoxicological information
Short-term toxicity to fish
Currently viewing:
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No analytical measurements of test material. TOC analyticals did not show gradient
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate)
- EC Number:
- 224-235-5
- EC Name:
- Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate)
- Cas Number:
- 4259-15-8
- Molecular formula:
- Too complex
- IUPAC Name:
- 1-Hexanol, 2-ethyl-, O,O-diester with phosphorodithioic acid, zinc salt
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water accommodated fraction (WAFs)
Test material was added to 21 L of water and stirred for 48 hours. The stir rate was set to form a slight dimple at the water surface. After stirring, the mixture was allowed to stand for 4 hours. A wide bore glass tube, covered at one end with Parafilm was submerged into the vessel, sealed end down, to a depth of approximately 5 cm from the bottom of the vessel. The aqueous phase or WAF was removed by mid-depth siphoning to give the necessary loading rates.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Microscopic inspection of the WAFs showed no micro-dispersions or undissolved test material
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Brow Well Fisheries Limited, Hebden, Yorkshire, UK
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 4.3 cm
- Weight at study initiation (mean and range, SD): 1.07 g
- Food type: Commerical trout pellets. Feeding was discontinued 24 hours prior to start of the study. Food was held for 24 hours.
ACCLIMATION
- Acclimation period: Two weeks
- Acclimation conditions (same as test or not): same as test conditions
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 100 mg/l as CaCO3
- Test temperature:
- 14 degrees C
- pH:
- 7.6 - 8.0
- Dissolved oxygen:
- 9.0 - 9.8 mg Oxygen/L
- Salinity:
- not applicable/freshwater
- Nominal and measured concentrations:
- Nominal: 1.0, 1.8, 3.2, 5.6, 10.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 20 litre glass vessels
- Aeration: None
- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated laboratory tap water passed through activated carbon filter
- Total organic carbon: 2 mg C/L
- Particulate matter: 0.10 mg/L
- Alkalinity: 104 mg/l as CaCO3
- Conductivity: 473 uS
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 1.0, 10, 100, and 1000 mg/L
- Results used to determine the conditions for the definitive study:
1.0 mg/L: 0/3
10, 100, and 1000 mg/L: 3/3 for each dose - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- 4.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 4.4 - 4.6 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Behavioural abnormalities: Loss of equilibrium in 5.6 mg/L Test group at 48 hours
- Mortality of control: 0/10
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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