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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Description of key information

The following information was chosen as the key study for this endpoint:
Hooftman R. N, de Wolf J. M. (2003). Dichlorodioctylstannane (CAS # 3542-36-7): Semi-static acute toxicity test with the crustacean species Daphnia magna. Testing laboratory: TNO, Project Organisation, Ecotoxicology, Utrechtseweg 48, P. O. Box 360, 3700 JA Zeist, The Netherlands. Report no.: 01-2497/02. Owner company: Organotin Environmental Programme (ORTEP) Association Stabilizer Task Force. Report date: 2003-09-15.
Hooftman & de Wolf, 2003, was chosen as the key study based upon the lack of deviations from the test guidelines (OECD guideline 202 and EU Method C.2) and the completeness of reporting. Although other sources of information were available these were considered not to be as reliable as the Hooftman & de Wolf, 2003, study.

Key value for chemical safety assessment

Additional information

The key study was selected on the basis that it was a 48 hour study conducted to the accepted OECD guideline for this endpoint, the study was conducted to GLP and the purity was reported as 99.91%. Due to the low solubility of the registered substance in water testing was conducted using test solutions prepared by the Water Soluble Fraction method. Test solutions with a nominal loading rate of 100mg/l were prepared by adding 2 litres of water to 200mg of test material and stirring mechanically for ca. 16 hours. Test solutions were analysed for test material concentration at 0, 24 and 48 hours. The geometric mean concentration was determined to be 0.28 mg/l, this concentration was above the known water solubility for the registered substance. As no immobilisation was reported in the study only a limit value of > 0.28 mg/l was estimated. It was therefore not possible to use this study for the purposes of classification and labelling.

Heer & Glaser, 1992, did report a definitive result; however this could not be used as the key study due to the use of a vehicle (ethanol) in the study.

Rufli, 1988, did not produce a definitive value due to the poor solubility of the test substance. This study was not performed to GLP and produced a higher EC50 value than the key study and therefore was not selected as a key study.

Steinhäuser et al, 1985, lacked information on methodologies and results and therefore could not be considered reliable enough to use as a key study.


In conclusion, the key acute daphnia 48 hour EC50 value is >0.28 mg/L. This cannot be used to classify the substance.