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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
genetic toxicity in vitro
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: acceptable data from a publication with solid information on materials/methodology and results

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Salmonella Mutagenicity Tests: II. Results From the Testing of 270 Chemicals
Author:
Mortelmans, K. et al.
Year:
1986
Bibliographic source:
Environ Mutagen 8, Supplement 7, 1-119
Reference Type:
publication
Title:
Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test.
Author:
Ames et al.
Year:
1975
Bibliographic source:
Ames BN, McCann J, Yamasaki E (1975); Mutat Res 31:347-364

Materials and methods

Principles of method if other than guideline:
In this publication of Mortelmans et al. is referred to various publications including the essential publication of Ames et al. (1975) to describe and justifiy materials and methodology that were applied.
GLP compliance:
not specified
Type of assay:
other: Salmonella/mammalian microsome assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name of the test substance as stated in the publication: diethyl carbonate
Purity: > 98 %
Purchased from: Aldrich

Method

Species / strain
Species / strain / cell type:
other: Salmonella TA1535, TA1537, TA98, TA100
Metabolic activation:
with and without
Metabolic activation system:
S9-mix, rat and hamster liver
Test concentrations with justification for top dose:
0, 100, 333, 1000, 3333, 6666 µg/plate
Vehicle / solvent:
Solvent dimethylsulfoxide (DMSO)
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: sodium azide (TA1535, TA100), 4-nitro-o-phenylenediamine (TA98), 9-aminoacridine (TA97, TA1537)

Results and discussion

Test results
Species / strain:
other: Salmonella TA1535, TA1537, TA98, TA100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test substance diethyl carbonate showed no mutagenicity on the different bacterial strains in the dose-level range from 0-6666 µg/plate.