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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on test solutions:
Stock solutions were prepared daily (1000 mg/l). Preparation by stirring with magnetic stirring bar for 1 h.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Source: Bio International B.V. (Netherlands)
- Date of birth: 27.05.1997
- Date of delivery: 15.07.1997
- Length at study initiation (length definition, mean, range and SD): 2,5 - 3,5 cm
- Mortality at keeping before test: < 2 %
- Medical pretreatment: none
- Keeping water: synthetic freshwater according to ISO
Test type:
flow-through
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
13.7 °dH
Test temperature:
control: 21.6 °C (0 h), 21.7 (24 h), 21.9 (48 h), 22.0 (72 h), 22.1 (96 h)
25 mg/l: 21.6 °C (0 h), 21.9 (24 h), 22.2 (48 h), 22.3 (72 h), 22.3 (96 h)
50 mg/l: 21.6 °C (0 h), 22.1 (24 h), 22.3 (48 h), 22.4 (72 h), 22.4 (96 h)
100 mg/l: 21.5 °C (0 h), 22.0 (24 h), 22.2 (48 h), 22.3 (72 h), 22.4 (96 h)
pH:
control: 8.2 (0 h), 8.0 (24 h), 8.0 (48 h), 8.0 (72 h), 8.1 (96 h)
25 mg/l: 8.1 (0 h), 8.0 (24 h), 8.0 (48 h), 8.0 (72 h), 8.0 (96 h)
50 mg/l: 8.0 (0 h), 8.0 (24 h), 8.0 (48 h), 8.0 (72 h), 8.0 (96 h)
100 mg/l: 8.1 (0 h), 8.0 (24 h), 8.0 (48 h), 7.9 (72 h), 7.9 (96 h)
Dissolved oxygen:
control: 8.8 mg/l (0 h), 8.9 mg/l (24 h), 8.7 mg/l (48 h), 8.9 mg/l (72 h), 8.9 mg/l (96 h)
control, dissolved oxygen saturation: 101.8 % min. (48 h) - 104.5 % max. (96 h)
25 mg/l: 8.4 mg/l (0 h), 8.9 mg/l (24 h), 8.9 mg/l (48 h), 8.8 mg/l (72 h), 8.8 mg/l (96 h)
25 mg/l, dissolved oxygen saturation: 97.8 % min. (0 h) - 104.7 % max. (48 h)
50 mg/l: 8.4 mg/l (0 h), 8.8 mg/l (24 h), 8.7 mg/l (48 h), 8.7 mg/l (72 h), 8.7 mg/l (96 h)
50 mg/l, dissolved oxygen saturation: 97.8 min. (0 h) - 103.3 max. (24 h) %
100 mg/l: 8.7 mg/l (0 h), 9.0 mg/l (24 h), 9.0 mg/l (48 h), 8.9 mg/l (72 h), 9.0 mg/l (96 h)
100 mg/l, dissolved oxygen saturation: 101.0 % min. (0 h) - 106.3 % max. (96 h)
Nominal and measured concentrations:
Nominal: 0 (control), 25, 50 and 100 mg/l

Measured, control: < 2 mg/l (0 h), < 2 mg/l (24 h), < 2 mg/l (28 h), < 2 mg/l (48 h)
Measured, 25 mg/l: 12 mg/l (0 h), 4 mg/l (24 h), 4 mg/l (28 h), 2 mg/l (48 h)
Measured, 50 mg/l: 23 mg/l (0 h), 8 mg/l (24 h), 10 mg/l (28 h), 7 mg/l (48 h)
Measured, 100 mg/l: 46 mg/l (0 h), 24 mg/l (24 h), 40 mg/l (28 h), 20 mg/l (48 h)
Details on test conditions:
TEST SYSTEM
- Test vessel: test aquariums 300 x 135 x 200 mm
- Test medium volume: 5 l
- Aeration: yes
- technical aids/solubiliser: magnetic stirring bar
- Renewal rate of test solution (frequency/flow rate): 6 times/day
- No. of organisms per vessel: 10

OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
>= 65 mg/L
Nominal / measured:
meas. (arithm. mean)
Details on results:
Mortality, cumulative value (number of dead fish after 96 h): 0 (control)
Mortality, cumulative value (number of dead fish after 96 h): 0 (25 mg/l)
Mortality, cumulative value (number of dead fish after 96 h): 0 (50 mg/l)
Mortality, cumulative value (number of dead fish after 96 h): 0 (100 mg/l)

Visually noticable effects on behaviour of fish:
At all test concentrations (control, 25, 50 and 100 mg/l) all fish out of a total of 10 showed normal swimming behaviour after 2, 24, 48, 72 and 96 h.

Fresh weight of 10 fish after killing at the end of the 96 h test period [g]: 1.65 g (control), 1.88 g (25 mg/l), 1.73 mg/l (50 mg/l), 1.58 mg/l (100 mg/l)
Validity criteria fulfilled:
yes
Conclusions:
No fish died.
LC0 (96 h) >= 65 mg/L (arithmetic mean of measured concentration)
(LC0 (96 h) >= 100 mg/L (if nominal concentration would be considered))

Description of key information

Determined LC0 in short-term-toxicity study to fish was ≥ 65 mg/L (nominal concentration ≥ 100 mg/L).

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

The authors of the short-term-toxicity study to fish evaluated a LC0 for the test substance diethyl carbonate. The highest nominal test concentration was 100 mg/L and according to the arithmetic mean of the analytically determined concentrations a LC0 ≥ 65 mg/L was evaluated for the test substance diethyl carbonate as no animals died during the 96 h incubation period. Based on these results and regarding that the highest nominal test concentration was 100 mg/L the author of this IUCLID data set assumed, that the LC50 value of the test substance should at least be 100 mg/L.