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Toxicity to microorganisms

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Description of key information

The effect of the test item on the respiration of activated sewage sludge gave a 3‑Hour EC50value of 58mg/l, 95% confidence limits 42-81mg/l. 

Key value for chemical safety assessment

EC50 for microorganisms:
58 mg/L

Additional information

A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2010) No 209 "Activated Sludge, Respiration Inhibition Test".

 Following a preliminary range-finding test activated sewage sludge was exposed to an aqueous dispersion of the test item at concentrations of10,32,100,320 and 1000mg/l (3 replicates) for a period of 3 hours at a temperature of 20±2°C with the addition of a synthetic sewage as a respiratory substrate.

The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-dichlorophenol.

The effect of the test item on the respiration of activated sewage sludge gave a 3‑Hour EC50value of 58mg/l, 95% confidence limits 42-81mg/l. 

The reference item gave a 3-Hour EC50value of 12mg/l, 95% confidence limits 10-15 mg/l.