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Type of information:
other: Category approach
Adequacy of study:
weight of evidence
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Density data have been generated for various protein families. The data published in the cited book comes from 20 different protein families and they all have densities in the range from 1.32 g/mL to 1.42 g/mL. The density for Subtilisins has not been determined but since it is a protein it is expected, with very high reliability, that also Subtilisins have density in this range.
Principles of method if other than guideline:
Determination of the sedimentation constant allows calculation of the density using the Svedberg equation. This has been performed in peer reviewed articles and summarised in Creighton "Proteins: structure and molecular properties" (1993) .
GLP compliance:
not specified
Key result
> 1.32 - < 1.42 g/cm³
20 °C
Remarks on result:
other: density range for 20 different proteins from different protein-families

Creighton reviews density data retrieved from structurally and fuctionally different proteins. They all have densities in the range from 1.32 to 1.42 g/mL, we are thus certain that also subtilisins have density in this range even though not experimentally determined.

Subtilisins are proteins and therefore it can be assumed that this group of enzymes has density within the range 1.32 and 1.42 g/mL.
Executive summary:

Densities have been determined for a wide range of different proteins. This analysis showed that the density range is between 1.32 and 1.42 g/mL. Since subtilisin is a protein, we conclude that this protein also has density within this range.

Description of key information

The densities have been determined for a wide range of different proteins, and this analysis showed that the density range is between 1.32 to 1.42 g/mL. Since subtilisins are proteins we assume that also these proteins have densities within this range. 

Key value for chemical safety assessment

Relative density at 20C:

Additional information

We report the average value of 1.37 g/mL as a representative value of the density. It should however be noted that the uncertainty is expected to be in the order of +/- 0.05 g/mL.