o-phenylenediamine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study but without GLP and deficiencies in documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: Bestimmung der Hemmwirkung von Wasserinhaltsstoffen auf Grünalgen Scenedesmus-Zellvermehrungs-Hemmtest L9 Stand 1987

Deviations:

not specified

Principles of method if other than guideline:

Bestimmung der Hemmwirkung von Wasserinhaltsstoffen auf Grünalgen Scenedesmus-Zellvermehrungs-Hemmtest L9 Stand 1987

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

o-phenylenediamine

EC Number:

202-430-6

EC Name:

o-phenylenediamine

Cas Number:

95-54-5

Molecular formula:

C6H8N2

IUPAC Name:

benzene-1,2-diamine

Test solutions

Vehicle:

not specified

Details on test solutions:

100 mg/l
32 mg/l
10 mg/l
3.2 mg/l
1.0 mg/l
0.32 mg/l
Kontrolle

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Secenedesmus subspicatus CHODAT - constant storage as test culture in Labour for water biology (WV-LE UWS)
starting concentration for the pre-culture: 10.000 cells / ml
testing: 10.000 cells/ml

Study design

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

3 d

Test conditions

Details on test conditions:

The test was done in Erlenemyerkolben (300 ml) with 100 ml test medium.
The storage was done in a "lighted board" with shaking equipment
Daily check of population growth of the one-cellulare algae over a period of 3 days

Results and discussion

Effect concentrations

Duration:

3 d

Dose descriptor:

EC50

Effect conc.:

4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Details on results:

test concentration % blocking of growth rate (control = 0%)
control 0
100 mg/l 107.6
32 mg/l 107.6
10 mg/l 75.9
3.2 mg/l 44.2
1.0 mg/l 26.0
0.32 mg/l 14.0

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The EC 50 of the test item on growth inhibition on Scenedesmus suspicatus was calculated graphically as 4.0 mg/l.

Executive summary:

.