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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study but without GLP and deficiencies in documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
other: Bestimmung der Hemmwirkung von Wasserinhaltsstoffen auf Grünalgen Scenedesmus-Zellvermehrungs-Hemmtest L9 Stand 1987
Deviations:
not specified
Principles of method if other than guideline:
Bestimmung der Hemmwirkung von Wasserinhaltsstoffen auf Grünalgen Scenedesmus-Zellvermehrungs-Hemmtest L9 Stand 1987
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine

Test solutions

Vehicle:
not specified
Details on test solutions:
100 mg/l
32 mg/l
10 mg/l
3.2 mg/l
1.0 mg/l
0.32 mg/l
Kontrolle

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
Secenedesmus subspicatus CHODAT - constant storage as test culture in Labour for water biology (WV-LE UWS)
starting concentration for the pre-culture: 10.000 cells / ml
testing: 10.000 cells/ml

Study design

Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
3 d

Test conditions

Details on test conditions:
The test was done in Erlenemyerkolben (300 ml) with 100 ml test medium.
The storage was done in a "lighted board" with shaking equipment
Daily check of population growth of the one-cellulare algae over a period of 3 days

Results and discussion

Effect concentrations
Duration:
3 d
Dose descriptor:
EC50
Effect conc.:
4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
cell number
Details on results:
test concentration % blocking of growth rate (control = 0%)
control 0
100 mg/l 107.6
32 mg/l 107.6
10 mg/l 75.9
3.2 mg/l 44.2
1.0 mg/l 26.0
0.32 mg/l 14.0

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC 50 of the test item on growth inhibition on Scenedesmus suspicatus was calculated graphically as 4.0 mg/l.
Executive summary:

.

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