Registration Dossier
Registration Dossier
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EC number: 429-270-1 | CAS number: 136210-30-5
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The chronic toxicity of aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester towards reproduction of Daphnia magna was tested according to the EC-method C.20, No. 440/2008 yielding the following results (Currenta, 2009d):
21d-NOEC (reproduction): 0.013 mg/L
21d-LOEC (reproduction): 0.041 mg/L
21d-NOEC (mortality): >= 0.12 mg/L
21d-LOEC (mortality): > 0.12 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.013 mg/L
Additional information
The results are expressed in terms of the geometric mean of measured concentrations. Recovery rates ranged from 23.5 - 78.1 % of the nominal values in the freshly prepared media and from 3.2 - 6.7 % in the media after 48 hours, whereas the hydrolysis of the test item is assumed as reason for the declining concentrations.
Study was performed with aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester which is a structural analogue to aspartic acid, N,N'-(methylenedi-4,1-cyclohexanediyl)bis-, 1,1',4,4'-tetraethyl ester. Both substances are diethyl esters of aspartic acid linked to a dicyclohexylmethyldiamine moiety. The difference between these two substances is merely the presence of two methyl groups connected to the cyclohexane rings. This structural analogy was confirmed by the Member State responsible for the notification of both substances under the NONS regulation. The Member State decided that test results obtained for one substance can be transferred to the other substance and that testing of both substances is usually not required. This decision is in accordance with the grouping of substances and read-across approach in Annex XI, 1.5 of the REACH Regulation.
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