Registration Dossier

Administrative data

Key value for chemical safety assessment

Additional information

Methyl chloroformate was tested in the pre incubation assay according to OECD TG 471 with and without metabolic activation in S.typhimuriumTA98, TA100, TA1535 and TA1537 at dose levels of 5 -5000 µg /plate. No increase in the number of revertants was detected in any strain with and without metabolic activation. Vehicle controls and positive controls were valid. Cytotoxicity was observed (concentrations not stated). Methylchloroformate was not mutagenic in this assay (BASF AG, 1988).

Methylchloroformate was tested for chromosomal aberrations in a study comparable to OECD TG 473, with V79 cells at concentrations of 0.1, 1.0, 2.5 µl/mL. Positive and negative controls reacted appropriately. Cytotoxicity was observed (concentration not stated). Under the conditions described in this report, there was an unequivocal enhancement of the aberration rates 7 h and 28 h after the treatment of the cells with 2.5 µ1/ml in the presence of metabolic activation but the results were negative after 18 hours. No chromosomal aberrations were observed in the absence of metabolic activation. Methylchloroformate is considered ambigous for chromosomal aberrations in the presence of metabolic activation (LMP, 1986).

Short description of key information:
Methylchloroformate was not mutagenic in the Ames test. Ambiguous results were obtain in a chromosomal aberration as positive and negative results for chromosomal aberrations in the presence of metabolic activation. Ambiguous results are assumed to be due to cytotoxicity and/or deliberation of hydrochloric acid of methylchloroformate in the test system..

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

No classification concerning repeated dose toxicity is warranted according to EU Regulation 67/548 and EU Regulation 1272/2008 as classification criteria are not met.