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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Additional information on environmental fate and behaviour

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Administrative data

Endpoint:
additional information on environmental fate and behaviour
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study meets generally accepted scientific methods and is described in sufficient detail.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Acute toxicity, critical body residues, Michaelis-Menten analysis of bioaccumulation, and ionoregulatory disturbance in response to waterborne nickel in four invertebrates: Chironomus riparius, Lymnaea stagnalis, Lumbriculus variegatus and Daphnia pulex.
Author:
Leonard EM, Wood CM
Year:
2013
Bibliographic source:
Comp Biochem Physiol C Toxicol Pharmacol. 2013 Jun;158(1):10-21. doi: 10.1016/j.cbpc.2013.03.008. Epub 2013 Apr 6.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Kinetics were measured for four aquatic invertebrate species
GLP compliance:
no
Type of study / information:
Kinetics

Test material

Constituent 1
Reference substance name:
Nickel chloride
EC Number:
253-399-0
EC Name:
Nickel chloride
Cas Number:
37211-05-5
IUPAC Name:
nickel(2+) dichloride
Test material form:
other: stock solutions
Details on test material:
- Name of test material (as cited in study report): Ni emily checking with authors

Results and discussion

Any other information on results incl. tables

In comparison to the other organisms, L. stagnalis had the highest affinity (lowest Kd value) and a relatively low capacity (low Bmax) for Ni. Kd values were next lowest for D. pulex, followed by L. variegatus and finally C. riparius with exceptionally high values. After L. stagnalis, Bmax values were slightly greater in L. variegatus, much greater in D. pulex, and again extremely high in C. riparius. In

general Bmax values tended to be higher in SW than in HW, while there was no clear effect of hardness on Kd values; the only significant hardness-related difference was the 2.8 fold higher Bmax value in SW vs. HW chironomids. In comparison to the other organisms, C. riparius had by far the highest Kd or Bmax values, indicating the lowest affinity and highest capacity. Whole body Ni bioaccumulation followed Michaelis-Menten kinetics in all organisms, with greater hardness tending to decrease Bmax with no consistent effect on Kd.

Michaelis-Menten kinetic constants (Bmax and Kd) for saturable Ni bioaccumulation in Daphnia pulex, Lymnaea stagnalis, Lumbriculus variegatusandChironomus riparius in hard water (HW, nominally 140 mg/L as CaC03) and soft water (SW, nominally 40 mg/L as CaC03).

 

Bmax(µmol/kg wet wt)

Kd(µmol Ni/L)

r2

Lymnaea stagnalis

 

 

 

Soft water

177.8 ± 30.1

2.5 ± 1.1

0.97

Daphnia pulex

 

 

 

Hard water

1522.3 ± 300.0

18.4 ± 9.7

0.97

Soft water

2015.9 ± 552.2

56.5 ± 26.9

0.98

Lumbriculus variegatus

 

 

 

Hard water

226.0 ± 47.6

133.9 ± 69.4

0.93

Soft water

441.5 ± 152.8

128.2 ± 85.8

0.95

Chironomus riparius

 

 

 

Hard water

24,496.5 ± 4167.3

1771.7 ± 1183.9

0.80

Soft water

68,404.1 ± 9839.8*

3619.0 ± 1425.2

0.95

* Indicates a significant difference in kinetic constants between SW and HW. Values are means ± S.E.M.

Applicant's summary and conclusion

Executive summary:

STUDY RATED BY AN INDEPENDENT REVIEWER