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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented study similar to OECD 403 guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1985
Report date:
1985

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
not specified
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzenesulphonyl chloride
EC Number:
202-636-6
EC Name:
Benzenesulphonyl chloride
Cas Number:
98-09-9
Molecular formula:
C6H5ClO2S
IUPAC Name:
benzenesulfonyl chloride
Details on test material:
- Name of test material (as cited in study report): Benzenesulfonyl Chloride
- Molecular formula (if other than submission substance): C6H5ClO2S
- Molecular weight (if other than submission substance): 176.62
- Physical state: Brown liquid

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: purchased from Charles River Laboratories (Kingston, NY)
- Age at study initiation: 9 weeks
- Weight at study initiation: males ca. 285 g and females ca. 189 g
- Fasting period before study: no
- Housing: housed singly in 7" wide x 9.5" deep x 7" high stainless steel wire bottom cages (Lab Products, Inc., Rochelle Park, NJ)
- Diet (e.g. ad libitum): Purina Certified Laboratory Rodent Chow #5002, Ralston Purina, St. louis, MO : ad libitum
- Water (e.g. ad libitum): Tap water ad libitum
- Acclimation period: 12 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 °C
- Humidity (%): 40% - 60%
- Air changes (per hr): 12.6
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:
inhalation: aerosol
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: inhalation chamber obtained from a commercial supplier (Young and Bertke Co., Cincinnati, OH) and is similar to the EPA design described by Hinners and coworkers. The chamber is constructed of stainless steel and glass.
- Exposure chamber volume: 447 liters
- Method of holding animals in test chamber: individualy caged in a compartmented stainless steel wire cage (5" x 8" x 7")
- Source and rate of air: conditioned room aire passed through an absolute filter
- System of generating particulates/aerosols: Solosphere nebulizer (Miller, et al., 1981)
- Method of particle size determination: by a low volume cascade impactor (Aries, Inc., Davis, CA)
- Treatment of exhaust air: passed through an absolute filter to remove particulate contaminants, and through an activated charcoal element to remove vapor contaminants, then further purified by the building exhaust system before release.


TEST ATMOSPHERE
- Samples taken from breathing zone: yes


TEST ATMOSPHERE (if not tabulated)
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): MMAD of 4.40µm with GSD of 1.18
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
1 h
Concentrations:
The mean total chamber air concentration of BSC was 1.87 mg/l with a standard deviation of +- 0.10. The calculated nominal concentration was 6.85 mg/l.
No. of animals per sex per dose:
5 male and 5 female
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Rats were observed frequently during exposure. After exposure, the rats will be observed twice a day and all clinical signs and mortalities will be recorded.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight: body weights will be recorded on day 0, 7 and 14.
Statistics:
Body weights wil be compared to their day 0 weights to check for significant weight gain or depression using Student's paired T-test

Results and discussion

Mortality:
A one-hour inhalation exposure of rats to 1.87 mg/l BSC caused 9 death (90%)
Clinical signs:
other: Reduced activity, wet muzzles, dyspnea/wheezing, dacryorrhea, chromodacryorrhea, chromorhinorrhea, brown flacks on chin and face, wet pelvic/ventral areas and some hair loss.
Other findings:
Necropsy findings suggest death was due to pulmonary insufficiency evidenced by pulmonary congestion.

Any other information on results incl. tables

No endpoint has been calculated ; A one-hour inhalation exposure of rats to 1.87 mg/l BSC caused 9 death (90%)

Applicant's summary and conclusion