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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

Conclusions are based on read-across from analogue substances of a known category. At a concentration of 100 mg/l, i. e. >> water solubility, the substance (EC# 230-743-8) is not expected to be toxic to waste water (activated sludge) bacteria: NOEC ≥100 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information

Read across to structural analogue. All members of this category of esters have a hydrophobic nature. Based on structural similarities of the substance of interest and the members of the category it was concluded that it was justified to applyread across for this endpoint.


Toxicity to microorganisms - HATCOL 3331

The influence of HATCOL 3331 on the respiration rate of activated sludge was investigated after a contact time of 30 minutes.

Since HATCOL 3331 was hardly soluble in water, the test substance was quantitatively added to the test vessels. A nominal concentration of 100 mg/L was tested in duplicate. The concentration was approved by the study director in the study files.

No significant inhibition of respiration rate of the sludge was recorded at 100 mg HA TCOL 3331 per litre. The duplicate measurement confirmed the result of the first measurement. Therefore no further testing was needed.

The respiration rates of the controls were within 15% of each other.

The EC50 of the reference substance, 3,5-dichlorophenol, was 11 mg/L.

Therefore, the test was considered to be valid.

In conclusion, under the conditions of this present test, HATCOL 3331 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/L.