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EC number: 200-526-2 | CAS number: 62-23-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The Zahn-Wellens test was conducted according to national standard (DIN 38 412, Part 25, 1984), which is similar to OECD 302B; limited documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- yes
- Remarks:
- In the today's methods no industrial but only municipal wastewater sludge is used and the concentrations of test substance and sludge are lower
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 4-nitrobenzoic acid
- EC Number:
- 200-526-2
- EC Name:
- 4-nitrobenzoic acid
- Cas Number:
- 62-23-7
- Molecular formula:
- C7H5NO4
- IUPAC Name:
- 4-nitrobenzoic acid
Constituent 1
Study design
- Oxygen conditions:
- not specified
- Inoculum or test system:
- activated sludge, industrial (adaptation not specified)
- Duration of test (contact time):
- 20 d
Initial test substance concentration
- Initial conc.:
- ca. 3 g/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Preparation of the stock solution: 5 g/L test substance was stirred for 24 h in deionized water to prepare a saturated solution. The acidic pH was adjusted with 1 m NaOH solution from 3.4 to 7.0. The resulting suspension was filtered through a folded paper filter, the filtrate was used as stock solution.
DOC of the stock solution: 955 mg/L
COD of the stock solution: 4180 mg/L
Reference substance
- Reference substance:
- diethylene glycol
- Remarks:
- 271.6 mg DOC/L
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 3 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 37
- Sampling time:
- 6 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 94
- Sampling time:
- 9 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 95
- Sampling time:
- 20 d
BOD5 / COD results
- Results with reference substance:
- 1 d: 6 %
3 d: 44 %
6 d: 100 %
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The test item is inherently and completely biodegradable.
- Executive summary:
Testing of 4 -nitrobenzoic acid for inherent biodegradability was performed in the Zahn-Wellens test. The results of testing revealed that the substance is inherently and completely biodegradable. The degradation of the reference substance was in the demanded range and caused a DOC reduction of 100 % within 6 days.
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