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Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012-02-06 to 2012-02-23
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Version / remarks:
August 24, 2009
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
adopted March 23, 2006, corrected July 28, 2011
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
- Name of test material (as cited in study report): Triethyleneglycol dimethacrylate
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 6.25, 12.5, 25, 50, 100 mg/L
- Sampling method: duplicate samples from the test media of all test concentrations and the control (containing algae) were taken at the end of the test (after the 72 hours test period)
- Sample storage conditions before analysis: freezer (≤ - 10 °C), protected from light
Vehicle:
no
Details on test solutions:
- Method: test medium of the highest test concentration of nominal 100 mg/L was prepared by dissolving 50.5 mg test item into 505 mL test water by intense stirring for 20 minutes. Adequate volumes of this test medium were diluted with test water to prepare the test media of the other desired test concentrations.
- Controls: test water without test item
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no remarkable observations
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: green algae
- Strain: 61.81 SAG
- Source (laboratory, culture collection): "Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen", 37073 Göttingen, Germany
- Age of inoculum (at test initiation): 3 days pre-culture
- Method of cultivation: in the laboratories of IBACON under standardised conditions according to the test guidelines

ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same conditions as in test
- Any deformed or abnormal cells observed: no data
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
no
Hardness:
0.24 mmol/L CaCO3
Test temperature:
23 to 25°C
pH:
7.7 to 8.1 at test start and 8.5 to 10.2 at test end
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
nominal 100, 50, 25, 12.5 and 6.25 mg/L, corresponding to 92.2, 44.1, 18.6, 9.05 and 4.52 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks of 50 mL volume with 50 mL of test medium
- Aeration: continuous stirring
- Initial cells density: 5000 cells/mL test medium
- Control end cells density: 117.825 ± 6.764 x10E4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes (OECD medium)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water, OECD medium
- Conductivity: <5 μS/cm

OTHER TEST CONDITIONS
- Adjustment of pH: adjustment to to pH 8.1
- Photoperiod: continuous illumination
- Light intensity and quality: 6600 lux (range: 5800 to 7670 lux)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometer; 24, 48, 72 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: 5 concentrations used as recommended
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
18.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
61 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL 53.4 - 67.1 mg/L
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
18.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
22 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 95% CL 12.6 - 29.6 mg/L
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
72.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 95%CL 61.6 - 90.1 mg/L
Details on results:
- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: at nominal 100 mg test item/L, the cells were deformed after 72h test duration; no differences at 50 mg/L
- Effect concentrations exceeding solubility of substance in test medium: no
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50: 72 h ErC50 1.073 mg/L (95% C.I. 1.009 – 1.143), 72 h EyC50 0.445 mg/L (95% C.I. 0.388 – 0.510)
Reported statistics and error estimates:
72-h ErC50, 72-h EyC50, the corresponding EC10 values and their 95 %-confidence limits: Probit analysis
72-h LOEC, 72-h NOEC, the calculated growth rates and yields at each test concentration were tested for significant differences compared to the control values by the Williams t-test (yield and growth rate), respectively
Validity criteria fulfilled:
yes
Conclusions:
The influence of Triethyleneglycol dimethacrylate on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. The 72-hour ErC50 value was calculated to be > 100 mg/L and the 72-hour EyC50 was calculated to be 72.8 mg/L. The 72 h ECr10 was 61.0 mg/l, the 72 h Ey10 was 22 mg/L.
Executive summary:

In a 72 hour acute toxicity study, the cultures of Pseudokirchnerella subcapitata strain 61.81 SAG were exposed to Triethyleneglycol dimethacrylate at nominal concentrations of 0 (control), 6.25, 12.5, 25, 50 and 100 mg/L under static conditions in accordance with the OECD guideline 201, adopted March 23, 2006, corrected July 28, 2011.

At 100 mg/L the cells were deformed after 72h test duration. At 50 mg/L no difference to the control was observed.

The NOEC, EC10 and EC50 values based on growth rate were 18.6, 61.0 mg/L (95% CL: 53.4 - 67.1 mg/L) and > 100 mg/L, respectively.

The NOEC, EC10 and EC50 values based on yield were 18.6, 22 and 72.8, respectively.

This toxicity study is classified as acceptable and satisfies the guideline requirements for a Freshwater Alga and Cyanobacteria toxicity study.

Results Synopsis

Test Organism: Pseudokirchnerella subcapitata

Test Type: Static

72 h ErC10: 61.0 mg/L 95% CL: 53.4 to 67.1 mg/L

72 h ErC50: >100 mg/L

72 h NOEC: 18.6 mg/L

Endpoint Effected: growth rate

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

The 72 h EC10 for TREGDMA in freshwater algae was 61.0 mg/L. The 72 h EC50 for TREGDMA in freshwater algae was >100 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L
EC10 or NOEC for freshwater algae:
61 mg/L

Additional information

One study in freshwater algae is available for TREGDMA, which is considered reliable without restriction (Klimisch score 1).

In a 72 hour acute toxicity study, the cultures of Pseudokirchnerella subcapitata strain 61.81 SAG were exposed to Triethyleneglycol dimethacrylate at nominal concentrations of 0 (control), 6.25, 12.5, 25, 50 and 100 mg/L under static conditions in accordance with the OECD guideline 201, adopted March 23, 2006, corrected July 28, 2011.

At 100 mg/L the cells were deformed after 72h test duration. At 50 mg/L no difference to the control was observed.

The NOEC, EC10 and EC50 values based on growth rate were 18.6, 61.0 mg/L and > 100 mg/L, respectively.