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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
17th July 1992
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
- Name of test material (as cited in study report): Triethylene glycol dimethacrylate
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0(control), 9.5, 17.2, 30.9, 55.6, 100.0 mg/L
- Sampling method: concentrations checked at test start + in 24 h old medium
- Sample storage conditions before analysis: no data
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: substance was weighed directly
- Controls: purified drinking water
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebra fish
- Strain: Hamilton-Buchanan
- Source: West Aquarium GmbH, Bad Lauterberg
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 2.2 to 2.9 cm, mean 2.5 ± 0.5 cm
- Weight at study initiation (mean and range, SD): 0.11 to 0.28 g, mean 0.20 ± 0.05 g
- Method of breeding: no data
- Feeding during test: no feeding during the test + 24 h before start of the test

ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): water of the same quality used for acclimatisation and test
- Type and amount of food: TetraMin
- Feeding frequency: daily
- Health during acclimation (any mortality observed): only healthy fish without diseases and abnormalities were used for the study
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no
Hardness:
no data
Test temperature:
21.8 to 22.3°C
pH:
8.0 to 8.3
Dissolved oxygen:
7.2 to 8.6 mg/L, saturation 85-101%
Salinity:
no data
Nominal and measured concentrations:
Nominal (measured mean ± SD):
0 (0.03 ± 0.07)
9.5 (8.92 ± 0.49)
17.2 (15.58 ± 0.98)
30.9 (29.32 ± 0.99)
55.6 (51.49 ± 2.82)
100.0 (88.16 ± 9.97)
Maximum relative deviation 11.8%
Details on test conditions:
TEST SYSTEM
- Test vessel
- Material, size, headspace, fill volume: 15 L all-glass aquaria, 10 L fill volume
- Aeration: yes, via glass capillary
- Renewal rate of test solution (frequency/flow rate): daily renewal
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified drinking water
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily (pH, oxygen, temperature)

OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: 14 h light, 10 h dark
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, abnormal behaviour (daily observation); dead animals were removed from the test vessels

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using less concentrations than requested by guideline: 5 concentrations were used as recommended
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
23.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 9.9 - 53.7 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
17.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 15.8 - 20.3 mg/L
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
17.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 15.2 - 19.4 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
16.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 14.5 - 18.6 mg/L
Details on results:
- Mortality of control: 0/10 died
- Effect concentrations exceeding solubility of substance in test medium: no (solubility 26 g/L)
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
Probit analysis

Nominal concentration [mg/L]

Cumulative mortality

24 h

48 h

72 h

96 h

0 (Control)

0

0

0

0

9.5

0

0

0

0

17.2

0

2

5

8

30.9

10

10

10

10

55.6

10

10

10

10

100.0

10

10

10

10

Validity criteria fulfilled:
yes
Conclusions:
In this 96-h acute toxicity study, Danio rerio (Zebra fish) were exposed to TREGDMA at nominal and measured concentrations of 0 (control), 9.5, 17.2, 30.9, 55.6 and 100.0 mg/L under static renewal conditions. The measured concentrations differed at maximum 11.8% from nominal concentrations.
The 96-h LC50 was 16.4 mg/L (based on nominal concentration).
Executive summary:

In a 96-h acute toxicity study according to OECD guideline 203, adopted 17th July 1992, Danio rerio (Zebra fish) were exposed to TREGDMA at nominal and measured concentrations of 0 (control), 9.5, 17.2, 30.9, 55.6 and 100.0 mg/L under static renewal conditions.

The measured concentrations differed at maximum 11.8% from nominal concentrations.

The 96-h LC50 was 16.4 mg/L (based on nominal concentration).

This toxicity study is classified as reliable without restriction and satisfies the guideline requirement for acute toxicity study to fish.

Results Synopsis

Test organism size/age (mean wet weight or length): mean length 2.5 ± 0.5 cm, mean weight 0.20 ± 0.05

Test Type: Static Renewal

LC50: 16.4 mgL 95% C.I.: 14.5 to 18.6 mg/L

NOEC: 9.5 mg/L

Endpoint(s) Effected: mortality

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

The 96 h-LC50 of TREGDMA in freshwater fish was 16.4 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
16.4 mg/L

Additional information

One acute freshwater study is available for TREGDMA, which is considered reliable without restriction (Klimisch score 1).

In a 96-h acute toxicity study according to OECD guideline 203, adopted 17th July 1992, Danio rerio (Zebra fish) were exposed to TREGDMA at nominal and measured concentrations of 0 (control), 9.5, 17.2, 30.9, 55.6 and 100.0 mg/L under static renewal conditions.

The measured concentrations differed at maximum 11.8% from nominal concentrations.

The 96-h LC50 was 16.4 mg/L (based on nominal concentration). The 96 h-NOEC was 9.5 mg/L.