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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N''-hexane-1,6-diylbis[N'-benzylurea]
EC Number:
254-274-3
EC Name:
N,N''-hexane-1,6-diylbis[N'-benzylurea]
Cas Number:
39072-70-3
Molecular formula:
C22H30N4O2
IUPAC Name:
N',N'''-hexane-1,6-diylbis(1-benzylurea)
Details on test material:
Description White powder (determined at NOTOX)
Batch 259-635
Purity > 90%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Frequency at t=0 h, t=24 h and t=96 h
Volume 2 ml from the approximate centre of the test vessels
Storage Samples were stored in a freezer until analysis

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Mean length 3.4 ± 0.1 cm
Mean weight 0.70 ± 0.10 g

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
180 mg CaCO3 per litre
Test temperature:
21.0 - 22.3°C
pH:
pH of 7.5 - 9.4

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
The 96h-LC50 exceeded 12 µg/l, being the mean measured concentration in a 0.45 µm filtered solution prepared at a loading rate of 100 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes