4-amino-6-chlorotoluene-3-sulphonic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Read-across from the structural isomer 5-Amino-2-chlorotoluene-4-sulphonic acid (CLT-acid)n CAS 88-53-9 in which the positions of chlorine and methyl are exchanged Read-across from supporting substance (structural analogues of category ¿quinacridone pigments¿) Source: Pigment Red 282 (909-082-0)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

5-amino-2-chlorotoluene-4-sulphonic acid

EC Number:

201-839-7

EC Name:

5-amino-2-chlorotoluene-4-sulphonic acid

Cas Number:

88-53-9

IUPAC Name:

2-amino-5-chloro-4-methylbenzenesulfonic acid

Test solutions

Vehicle:

no

Details on test solutions:

aqueous solution of test substance with small precipitation amounts

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.3 - 22-4 °C

pH:

6.2 - 8.1

Dissolved oxygen:

6.4 - 9.1 mg/l

Nominal and measured concentrations:

Nominal concentrations: 1 000, 3 500, 5 000, 7 100 and 10 000 mg/L.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

3 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

6 350 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Justification for CLT-acid as a read across substance for the short-term fish toxicity of 2B-acid:

The read across substance CLT-acid (CAS-no. 88 -53 -9) differs from the registered substance 2B-acid (CAS-no. 88 -51 -7) only by the exchanged positions of the methyl and the clorine substituents. All other structural elements and positions are identical.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

67/548/EEC: Acute aquatic toxicity: no classification
1272/2008/EC: Acute aquatic toxicity: no classification