1,2-dichlorobenzene

Bioaccumulation: terrestrial

Administrative data

Endpoint:

bioaccumulation: terrestrial

Type of information:

other: BUA report

Adequacy of study:

other information

Reliability:

other: BUA report

Rationale for reliability incl. deficiencies:

other: BUA report

Data source

Reference

Reference 1

Reference Type:

other: BUA report

Title:

Unnamed

Year:

1990

Reference 2

Reference Type:

publication

Title:

Speicherung chlorierter Rheinwasserschadstoffe im Fettgewebe von Ratten

Author:

Jacobs A, Blangetti M, Hellmund E

Year:

1974

Bibliographic source:

Vom Wasser, 43: 259-274

Reference 3

Reference Type:

publication

Title:

Speicherung einiger im Rheinwasser als Schadstoffe identifizierter organischer Verbindungen im Fettgewebe von Ratten

Author:

Jacobs A, Blangetti M, Hellmund E, Kölle W

Year:

1974

Bibliographic source:

In: Wasser- und abwasserchemische Untersuchungen 1972/73. Kernforschungszentrum Karlsruhe, Gesellschaft für Kernforschung mbH, Karlsruhe, (Ber.), KFK 1969 UF: 1-7

Reference 4

Reference Type:

publication

Title:

Chemical hygiene studies on organic halogen compounds. I. Transfer of chlorobenzenes into hen eggs.

Author:

Kazama M, Yamazoe R, Ito K, Mizuishi K, Nakamura Y, Totani T

Year:

1971

Bibliographic source:

Tokyo Toritsu Eisei Kenkyusho Kenkyu Nempo, 23: 93-100 (Pub. 1972); cited in: Chem Abstr, 79, 14121h (1973)

Materials and methods

Principles of method if other than guideline:

BUA report

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,2-dichlorobenzene

EC Number:

202-425-9

EC Name:

1,2-dichlorobenzene

Cas Number:

95-50-1

Molecular formula:

C6H4Cl2

IUPAC Name:

1,2-dichlorobenzene

Results and discussion

Any other information on results incl. tables

BUA report:

3 studies on the potential for bioaccumulation of 1,2-dichlorobenzene to terrestrial organisms were summarized in the assessment of the BUA report 53 (1990). The reliability was not assignable because references were only cited as secondary literature.

The storage of o-dichlorobenzene was studied in rats following oral administration with feed impregnated with a solution in vegetable fat. Accumulation was observed in the fatty tissue as described below. When o-dichlorobenzene was administered in concentrations of 2 and 4 mg/kg body weight per day for 4 weeks, the concentration in the fatty tissue was 15 times the dose in both cases. After three months at 4 mg/kg body weight per day, it was twenty five times the dose, without saturation ever having been reached. Investigation of the biological persistence of the stored o-dichlorobenzene revealed that, when 4 mg/kg body weight per day were administered for four weeks, the substance was present in approximately the same amount one week after cessation but was no longer detectable after three weeks.

Further studies involving lower doses but following the same regimen revealed that, 12 weeks after 0.4 and 0.8 mg/kg body weight were administered orally, the abdominal and renal fat of rats contained 2.5% and 2% respectively of the total administered dose. In the 0.4 mg group, roughly 0.8 mg of o-dichlorobenzene was stored in the fat per animal whereas around 1 mg per rat was found in the 0.8 mg group. No o-dichlorobenzene was detectable in the blood.

After intramuscular injection of dichlorobenzene (85 - 96% o- and 4 - 15% p-dichlorobenzene; CAS No. 25321 -22 -6) to chickens, the egg yolks were found to contain 0.4 - 0.6% of the administered dose of around 50 mg/kg body weight. The egg white, which contains 100 times less fat than the yolk (Cremer et al, 1984), did not contain any dichlorobenzene residues.

Applicant's summary and conclusion

Executive summary:

3 studies on the potential for bioaccumulation of 1,2-dichlorobenzene to terrestrial organisms were summarized in the assessment of the BUA report 53 (1990). The reliability was not assignable because references were only cited as secondary literature.

The storage of o-dichlorobenzene was studied in rats following oral administration with feed impregnated with a solution in vegetable fat. Accumulation was observed in the fatty tissue as described below. When o-dichlorobenzene was administered in concentrations of 2 and 4 mg/kg body weight per day for 4 weeks, the concentration in the fatty tissue was 15 times the dose in both cases. After three months at 4 mg/kg body weight per day, it was twenty five times the dose, without saturation ever having been reached. Investigation of the biological persistence of the stored o-dichlorobenzene revealed that, when 4 mg/kg body weight per day were administered for four weeks, the substance was present in approximately the same amount one week after cessation but was no longer detectable after three weeks.

Further studies involving lower doses but following the same regimen revealed that, 12 weeks after 0.4 and 0.8 mg/kg body weight were administered orally, the abdominal and renal fat of rats contained 2.5% and 2% respectively of the total administered dose. In the 0.4 mg group, roughly 0.8 mg of o-dichlorobenzene was stored in the fat per animal whereas around 1 mg per rat was found in the 0.8 mg group. No o-dichlorobenzene was detectable in the blood.

After intramuscular injection of dichlorobenzene (85 - 96% o- and 4 - 15% p-dichlorobenzene; CAS No. 25321 -22 -6) to chickens, the egg yolks were found to contain 0.4 - 0.6% of the administered dose of around 50 mg/kg body weight. The egg white, which contains 100 times less fat than the yolk (Cremer et al, 1984), did not contain any dichlorobenzene residues.