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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Readily biodegradable: 60% (O2 consumption) of 2,2'-[ethane-1,2-diylbis(oxy)]bisethyl diacetate after 28 d (OECD 301C)

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

One GLP-Guideline study according to OECD 301C is available investigating the ready biodegradability of 2,2'-[ethane-1,2-diylbis(oxy)]bisethyl diacetate (CAS 111-21-7; Clarke, 2012). The study was performed using a mixed culture of sewage sludge microorganisms from ten different sites containing domestic and industrial sewage plants, lakes, rivers and sea as inoculum. After incubation of 28 d the test substance reached 60% (mean) based on O2 consumption and 96% based on DOC removal. The much higher extent in biodegradation based on DOC removal was considered to be due to adsorption of the test item to the MITI sludge which was removed from the samples taken for DOC analysis. An analytical determination of test substance by GC/FID resulted in degradation below the Limit of Quantification (LOQ) of 0.04 mg/L. A complete degradation was not assumed because the same result was also measured in the test item vessels without inoculum. Thus, degradation by hydrolysis and/or adsorption to glassware is assumed. Nevertheless, DOC measurement is an unspecific method and the result might not reflect completely the biodegradation of the substance. Since the substance reached a biodegradation ≥ 60% based on the O2 consumption after 28 d it is considered to be readily biodegradable according to the OECD criteria.