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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
June 12, 1992 - January 7, 1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Study conducted equivalent to OECD 202 and GLP. Missing information on the age of the Daphnids. No accurate monitoring of the test concentrations and as there is no data reported about any results obtained with a reference substance, the sensitivity can not be checked. Also, missing information about number of animals at each test concentration and for the controls. Still regarded as a reliable study as the validity criteria can be verified and are fulfilled (and there is no definite data about the age of the Dahnids/about the sensitivity).
Qualifier:
according to
Guideline:
other: DIN 38412, Teil 11
Deviations:
not applicable
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
no data about the age of the Daphnids; no data about results obtained with a reference substance; no data about number of animals at each test concentration and for the controls and no accurate monitoring of the test concentrations
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
The nominal concentrations are expressed based on an incorrect purity of the substance. These concentrations are here corrected:
- Concentrations: 325, 1250 and 7500 mg/L (aqueous solution), at t=0, t=24 and t=48h
Vehicle:
no
Details on test solutions:
The stock solution was prepared by adding 10 g of the testmaterial (aqueous solution) to 100 mL water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: from own laboratory
No further details available
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
No data
Test temperature:
20 - 21 degrees Celsius
pH:
At t=24h: 7.8-8.2
At t=48h: 8.0-8.1
Dissolved oxygen:
At t=24h: 83-94%
At t=48h: 85-94%
Salinity:
Not applicable
Nominal and measured concentrations:
The nominal concentrations are expressed based on an incorrect purity of the substance. These concentrations are here corrected:
Nominal concentrations: 0, 225, 325, 450, 625, 900, 1250, 1750, 2500, 7500 and 25000 mg/L (aqueous solution)
Nominal concentrations: 0, 78.75, 113.75, 157.5, 218.75, 315, 437.5, 612.5, 875, 2625 and 8750 mg/L (expressed as solid content)
Details on test conditions:
TEST SYSTEM
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412, Teil II

No further details available.
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
255 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
(aqueous solution)
Basis for effect:
mobility
Remarks on result:
other: Aqueous solution with a solid content of approximately 35%
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
89 mg/L
Nominal / measured:
nominal
Conc. based on:
other: expressed as solid content
Basis for effect:
mobility
Details on results:
- Based on the DOC measurements at t=0; t=24 and t=48 h, it is assessed that the concentrations remained >80% nominal (details not provided in this summary)
- The irrelevance of the DOC measurements is supported by the fact that the measurements at t=0 are higher than the nominal DOC values as well as by the fact that DOC values increase during the test (which is easily understandable as biological activity probably causes this increase).
Results with reference substance (positive control):
Not applicable
Validity criteria fulfilled:
yes
Remarks:
(1) Immobility in control < 10% (0%); 2) Oxygen >3 mg/L (>7.8 mg/L; reported as 85-94 %)
Conclusions:
In an acute Daphnia Magna study, conducted in accordance with DIN 38412, Teil II, an 48h-EC50 of 89 mg/L (expressed as solid content) was determined. It should be noted that the age of the Daphnids is not provided.
Executive summary:

In accordance with DIN 38412, Teil II, an acute toxicity study was conducted with Daphnia Magna. The test type was static and there was analytical monitoring. For the control and for the test groups, 2 replicates were used. A reference substance was not tested. The 48h-EC50 of the substance was determined to be 89 mg/L (expressed as solid content). All the validity criteria were fulfilled.

Description of key information

In a limit test with Dahnia magna, a 48h-EC50 of greater than 100 mg/L was determined. In another acute Daphnia Magna study, conducted in accordance with DIN 38412, Teil II, an 48h-EC50 of 89 mg/L was determined. This lowest value is taken forward to the chemical safety assessment.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
89 mg/L

Additional information

In accordance with DIN 38412, Teil II, an acute toxicity study was conducted with Daphnia Magna. The test type was static and there was analytical monitoring. For the control and for the test groups, 2 replicates were used. The 48h-EC50 of the substance was determined to be 89 mg/L (expressed as solid content).