Registration Dossier
Registration Dossier
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EC number: 201-855-4 | CAS number: 88-74-4
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study described shortly but sufficient for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- No guideline followed
- GLP compliance:
- no
Test material
- Reference substance name:
- 2-nitroaniline
- EC Number:
- 201-855-4
- EC Name:
- 2-nitroaniline
- Cas Number:
- 88-74-4
- Molecular formula:
- C6H6N2O2
- IUPAC Name:
- 2-nitroaniline
- Details on test material:
- - Name of test material (as cited in study report): Nitrobenzene
- Analytical purity: no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- other: Scenedesmus obliquus
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 100 ml sterile flasks
- Initial cells density: 10E+04 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: no data
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no data
- Photoperiod: 12:12
- Light intensity and quality: 4000 Lux - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 64.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
