Diphenyl sulphone

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in mammalian cells

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets scientific and regulatory standards, but acceptable restrictions (non-GLP test article certification, stability of test article not analysed and no certification of purity and stability

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)

Deviations:

yes

Principles of method if other than guideline:

Minor modifications

GLP compliance:

yes

Type of assay:

mammalian cell gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Diphenyl sulphone

EC Number:

204-853-1

EC Name:

Diphenyl sulphone

Cas Number:

127-63-9

Molecular formula:

C12H10O2S

IUPAC Name:

diphenyl sulphone

Constituent 2

Reference substance name:

1,1'-sulfonyldibezene

IUPAC Name:

1,1'-sulfonyldibezene

Test material form:

solid: crystalline

Details on test material:

- Name of test material (as cited in study report): diphenyl sulphone
- Substance type: monoconstituent
- Physical state: white crystalline solid
- Analytical purity: stated purity 99.99%
- Impurities (identity and concentrations): see confidential details
- Composition of test material, percentage of components: 99,99% pure
- Isomers composition: not applicable
- Purity test date: see confidential details
- Lot/batch No.: see confidential details
- Expiration date of the lot/batch: see confidential details
- Stability under test conditions: stable under normal storage and test conditions according the Sponsor
- Storage condition of test material: at ambient temperature in the dark until required
- Other: not applicable

Method

Target gene:

Thymidine kinase TK +/- gene

Species / strain

Species / strain / cell type:

mouse lymphoma L5178Y cells

Details on mammalian cell type (if applicable):

-Type and identity of media: RPMI 1640 with Hepes (GIBCO) supplemented with 4mM L-glutamine (GIBCO), 200 units/mL of penicillin and streptomycin (GIBCO) and 10% horse serum (GIBCO). The serum concentration was dropped to 5% during treatment and raises to 20% whenever the cells were dispensed into microwells.
-Properly maintained: yes
-Periodically checked for Mycoplasma contamination: yes (by ELISA)
-Periodically checked for karyotype stability: no data
-Periodically”cleansed” against high spontaneous background: no data

Additional strain / cell type characteristics:

not applicable

Metabolic activation:

with and without

Metabolic activation system:

S9-mix

Test concentrations with justification for top dose:

Dose range finding experiment: 125; 63; 31; 16 µg/mL
Experiment 1, +S9 and –S9: 125; 63; 31; 16 µg/mL
Experiment 2, +S9 and –S9: 125; 63; 31; 16 µg/mL

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: the test material was found to be soluble in DMSO at all concentrations.

Controls

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

Positive controls:

yes

Remarks:

-S9:EMS (ethyl methanesulphonate 750 µg/mL); +S9: NDMA( N-nitrosodimethylamine 600 µg/mL)

Details on test system and experimental conditions:

METHOD OF APPLICATION: in medium (microwell plates)
DURATION
- Preincubation period: 1 hour
- Exposure duration: 4 hours
- Expression time (cells in growth medium): 72 hours
- Selection time (if incubation with a selection agent): 10-12 days

SELECTION AGENT (mutation assays): TFT ( trifluorothymidine)

NUMBER OF REPLICATIONS: duplicate

DETERMINATION OF CYTOTOXICITY
- Method: cloning efficiency; relative total growth; other: Viability scored by cell growth; mutation scored by colony count

Evaluation criteria:

Test data from individual experiments were considered valid if:
(a) the spontaneous mutant frequencies in the presence and absence of S9-mix fell within an acceptable range
(b) the positive controls induced unequivocal positive responses.

A positive response in a valid individual experiment is recorded when statistically significant, dose related increases in mutant frequency are observed across a range of toxicity. Such increases in mutant frequency are considered not to be indicative of a positive response if the increases occur only at doses eliciting excessive toxicity, ie <10% survival. These increases must also be associated with increases in the numbers of mutants over those observed with the concurrent solvent control.

A negative result in a valid experiment is obtained when there is no significant dose related increase in mutant frequency compared to the solvent control.

A positive response in an individual experiment must be reproducible for the test sample to be considered positive (ie mutagenic) in this assay.

Statistics:

A statistical analysis is applied at the discretion of the Study Director in consultation with the Study Statistician. In this study, the data were examined by the Study Statistician and Study Director who considered that statistical analyses were not necessary.

Results and discussion

Test results

Species / strain:

mouse lymphoma L5178Y cells

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Additional information on results:

The highest concentration was based on solubility of the test sample in culture medium, with 125 µg/mL being just in excess of the limit of solubility in the dose range finding study.

Remarks on result:

other: strain/cell type: L5178Y cells TK +/-

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Table 1. Summary of Data for Experiment 1

WITHOUT S9			WITH S9
Conc (µg/mL)	Mean % Survival	Mean Mutant Frequency (x10^-4)	Conc (µg/mL)	Mean % Survival	Mean Mutant Frequency (x10^-4)
DIPHENYL SULPHONE			DIPHENYL SULPHONE
125	116	2.8	125	125	1.4
63	120	1.9	63	149	1.4
31	109	2.5	31	124	1.4
16	119	2.2	16	118	1.4
SOLVENT CONTROL			SOLVENT CONTROL
DMSO (10µL/mL)	100	2.3	DMSO (10µL/mL)	100	1.9
POSITIVE CONTROL			POSITIVE CONTROL
EMS (750 µg/mL)	53	9.8	NDMA (600 µg/mL)	100	11.4

 

Table 2. Summary of Data for Experiment 2

WITHOUT S9			WITH S9
Conc (µg/mL)	Mean % Survival	Mean Mutant Frequency (x10^-4)	Conc (µg/mL)	Mean % Survival	Mean Mutant Frequency (x10^-4)
DIPHENYL SULPHONE			DIPHENYL SULPHONE
125	93	1.4	125	99	0.9
63	95	1.6	63	107	1.1
31	104	1.4	31	124	1.8
16	105	1.1	16	91	1.4
SOLVENT CONTROL			SOLVENT CONTROL
DMSO (10µL/mL)	100	1.4	DMSO (10µL/mL)	100	1.6
POSITIVE CONTROL			POSITIVE CONTROL
EMS   (750 µg/mL)	30	21.6	NDMA   (600 µg/mL)	73	19.9

 

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Under the conditions of this assay, diphenyl sulphone is non-mutagenic to L5178Y TK+/- cells as determined by selection in trifluorothymidine in both the presence and absence of an auxiliary metabolic activation system (S9-mix), when tested up to a concentration just in excess of the limit of solubility in the test medium.

Executive summary:

To assess the mutagenic potential of diphenyl sulphone to mammalian cells, L5178Y TK+/- mouse lymphoma cells were treated in vitro with various concentrations of test sample, both in presence and absence of a rat liver derived auxiliary metabolic activation system (S9-mix). Mutant frequencies were assessed by cell growth in the presence of trifluorothymidine (TFT) after a 72 hour expression time.

Two independent experiments were conducted using a range of concentrations of diphenyl sulphone in the presence and absence of S9-mix. The highest concentration of diphenyl sulphone tested in these two experiments was based on the solubility of the test sample in the culture medium, treatment with diphenyl sulphone at 125µg/mL being just in excess of the limit of solubility. No significant increases in the mutant frequency, compared to the solvent control values, were observed in either experiment in either the presence or absence of S9-mix.

It is therefore concluded that, under the conditions of this assay, diphenyl sulphone is non-mutagenic to L5178Y TK+/- cells in either the presence or absence of S9-mix when tested to a concentration just in excess of the limit of solubility.