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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation / corrosion
Remarks:
in vitro
Type of information:
experimental study
Adequacy of study:
key study
Study period:
04 September 2012 - 13 September 2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Conducted according to an appropriate OECD guideline and under GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report Date:
2012

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
- Name of test material (as cited in study report): tetrahydro-2-methylfuran

- Physical state: clear colourless liquid

- Storage condition of test material: room temperature in the dark under nitrogen

Test animals

Species:
other: n/a
Strain:
other: n/a
Details on test animals and environmental conditions:
n/a

Test system

Type of coverage:
other: n/a
Preparation of test site:
other: n/a
Vehicle:
unchanged (no vehicle)
Controls:
other: positive control: glacial acetic acid, negative control: 0.9% w/v sodium chloride solution
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 microlitres
- Concentration (if solution): undiluted

Duration of treatment / exposure:
3, 60 and 240 minutes.
Observation period:
The tissues were incubated in MTT solution overnight after stopping treatment, which after optical density measurements were taken.
Number of animals:
Duplicate tissue/test condition
Details on study design:
See 'any other information on materials and methods'.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: relative mean viability
Basis:
mean
Time point:
other: 3 minutes exposure
Score:
107.3
Reversibility:
other: n/a
Remarks on result:
other: Score expressed as a percentage
Irritation parameter:
other: relative mean viability
Basis:
mean
Time point:
other: 60 minutes exposure
Score:
57.3
Reversibility:
other: n/a
Remarks on result:
other: Score expressed as a percentage
Irritation parameter:
other: relative mean viability
Basis:
mean
Time point:
other: 240
Score:
79.1
Reversibility:
other: n/a
Remarks on result:
other: Score expressed as a percentage
Irritant / corrosive response data:
None reported.

Applicant's summary and conclusion

Interpretation of results:
other: Non-corrosive
Remarks:
Criteria used for interpretation of results: OECD GHS
Conclusions:
Tetrahydro-2-methylfuran was not considered to be corrosive to skin, as determined in an in vitro assay (EPISKIN) conducted in accordance with current OECD guideline and GLP.