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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Study period:
from 1990-07-30 to 1990-09-08
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: 1a: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Amines, bis(hydrogenated tallow alkyl)methyl
EC Number:
262-991-8
EC Name:
Amines, bis(hydrogenated tallow alkyl)methyl
Cas Number:
61788-63-4
IUPAC Name:
61788-63-4
Constituent 2
Reference substance name:
Ditallowmethylamine. hydrogenated
IUPAC Name:
Ditallowmethylamine. hydrogenated
Details on test material:
- Trade name of test material: Armeen M2HT
- Source: Akzo Chemicals BV
- Date of receipt: 1990-08-01
- Lot/batch No.: AT08/C-29.6.90
- Purity: 100%
- Physical state: solid
- Stability: stable at test concentrations
- Water solubility: practically insoluble
- Storageuntil used: at room temperature in the dark
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not relevant

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
no data

Test solutions

Vehicle:
no
Details on test solutions:
3 range-finding tests were investigated. The test concentrations of the third range-finding test and the definitive test were prepared by direct addition of the melted test substance to DSW at a temperature of 45°C under stirring.
After the fluid was cooled to a temprature below 30°C, the test substance solidified again and fat-like particles were formed.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Source: local aquariumretailer (Rasbora, Veenendaal
- Length at study initiation: 1.6 - 2.7 cm
- Weight at study initiation: 0.1 g

ACCLIMATION
- Acclimation period: at least 12 days
- Water: the water they were kept was Dutch Standard Water
- Feeding: 1 to 3 times each day (6 days a week), the last feeding taking place about 24h before the test was started.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
13°dH
Test temperature:
22.9 - 23.7°C
pH:
8.1 - 8.3
Dissolved oxygen:
8.4 - 8.8 mg O2/L
Salinity:
no data
Nominal and measured concentrations:
Nominal concentration: 1g/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 5L glass aquaria containing 3 L of test medium, which were covered with a glass plate.
- Biomass load of the test vessels = 0.23g of biomass/L.
- Aeration during the test: yes.
- No feeding during test.
- 7 fish in duplicate.
- Renewal: after 48h

TEST MEDIUM / WATER PARAMETERS
- Synthetic water, Dutch Standard Water
- pH of 8.2 and hardness of 13°dH.
- Containing per litre of deionized water:
-100 mg of NaHC03/1.
-20 mg of KHC03/1.
-200 mg of CaC12.2H2011
-180 mg of MgS04.7H20/1
- Conductivity: < 5 pS/c
- TOC content: < 2mg/L
- Copper: no more than 0.01 mg/L

OTHER TEST CONDITIONS
- Photoperiod: 12h of ambient light per day, supplied by fluorescent tubes
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Due to the practical insolubility of the test substance in water undissolved fat-like particles of the test substance were still present during the test.
- No chemical analysis of the actual dissolved test concentrations were performed. All concentrations mentioned are nominal concentrations and all results are related to them. Therefore, the results should be interpreted with care.
- All fish tested (2 times 7 fish) survived in the limit test during 96h in a test fluid containing 1g of the test substance/L. In this fluid solid particles were present.
- No other effects, such as deviations in the behaviour or the appearance of the fish, were observed.
Results with reference substance (positive control):
potassium dichromate: LC50-96h = 180 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
see conclusions
Conclusions:
Quality criteria:
- Mortality: the mortality of the control was <10%, criteria fulfilled.
- Oxygen concentration: >60% of the air saturation troughout the test, criteria fulfilled.
- The quality of the batch fish used in the test was further checked by means of a test with a reference substance (potassium dichromate).
The results obtained are in accordance with the test results of an EEC study mentioning a concentration of 205.5 mg/L as LC50-48h.
- Biodegradability: In view of the results of a biodegradability study, it is likely that the concentration of the test substance remained constant during the test.
Executive summary:

Acute toxicity of Amines, bis(hydrogenated tallow alkyl) methyl to Brachydanio rerio has been investigated through OECD Guideline 203 and EU Method C.1.

Brachydanio rerio were exposed for 96 hours to a solution of Amines, bis(hydrogenated tallow alkyl) methyl

at a limit nominal concentration of 1000 mg/L.

LC 50 - 96h was determined to be greater than 1000 mg/L.