Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-871-0 | CAS number: 624-92-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1998
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Version / remarks:
- 1998
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dimethyl disulphide
- EC Number:
- 210-871-0
- EC Name:
- Dimethyl disulphide
- Cas Number:
- 624-92-0
- Molecular formula:
- C2H6S2
- IUPAC Name:
- (methyldisulfanyl)methane
- Details on test material:
- - Physical state: light yellow liquid
- Analytical purity: 99.2%
- Purity test date: LJuly 21, 2007
- Lot/batch No.: VS060001
Constituent 1
Method
- Target gene:
- Histidine locus (Salmonella) and tryptophane locus (Escherichia)
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S9 homogenate was prepared from rats that have been induced with Arochlor 1254
- Test concentrations with justification for top dose:
- The dose levels tested were 1.5, 5.0, 15, 50, 150, 500, 1500 and 5000 µg per plate in the initial toxicity-mutation assay and 50, 150, 500, 1500 and 5000 µg per plate in the confirmatory mutagenicity assay.
- Vehicle / solvent:
- Dimethyl sulphoxide
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: With S9: 2-aminoanthracene, 1 µg/plate for all Salmonella strains, 10µg/plate for E. coli. Without S9: TA 98, 2-nitrofluorene (1 µg/plate); TA100 and TA 1535, sodium azide (1 µg/plate); TA 1537, 9-aminoacridine (75 µg/plate); E. coli, MMS (1000 µg/plate)
- Details on test system and experimental conditions:
- DETERMINATION OF CYTOTOXICITY
- Method: relative total growth (decrease in the number of revertant colonies and/or a thinning of the bacterial lawn);
EXPERIMENTS
- Initial toxicity-mutation assay: in all strains, with or without S9 mix ; 8 dose-levels (2 plates/dose level)
- Confirmatory mutagenicity assay: in all strains, with or without S9 mix ; 5 dose-levels (3 plates/dose level)
METHOD OF APPLICATION: Direct plate incorporation method: for preliminary both experiments
DURATION
- Exposure duration: 48-72H - Evaluation criteria:
- Reproducible increase in the number of revertant colonies (2-fold for TA98/TA100 and WP2 uvrA, 3-fold for TA 1535/TA 1537) compared with vehicle controls in any strain at any dose-level and/or evidence of a dose-relationship.
Reference to historical data and consideration to biological relevance may also be taken into account.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- Tested up to limit concentrations recommended by the test guideline
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING STUDY:
- Results on solubility: dimethyl disulphide formed a soluble and clear solution in dimethyl sulfoxide (DMSO) at approximately 500 mg/mL, the highest concentration tested.
- Results on cytotoxicity: In the initial toxicity-mutation assay, the maximum dose tested was 5000 µg/per plate; this dose was achieved using a concentration of 100 mg/mL and a 50 µL plating aliquot. The dose levels tested were 1.5, 5.0, 15, 50, 150, 500, 1500 and 5000 µg per plate. Neither precipitate nor appreciable toxicity was observed.
Any other information on results incl. tables
Number of revertants per plate (first experiment) (mean of 2 plates) |
||||||||||
|
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
WP2 uvrA |
|||||
Conc. [µg/plate] |
- MA |
+MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
+MA |
-MA |
+MA |
DMSO |
17 |
19 |
126 |
127 |
16 |
16 |
9 |
5 |
11 |
12 |
1.5 |
21 |
21 |
122 |
131 |
14 |
13 |
6 |
10 |
7 |
13 |
5.0 |
15 |
21 |
101 |
137 |
14 |
18 |
7 |
8 |
8 |
13 |
15 |
19 |
21 |
103 |
142 |
14 |
21 |
2 |
7 |
10 |
9 |
50 |
14 |
24 |
103 |
124 |
10 |
14 |
7 |
6 |
10 |
14 |
150 |
13 |
20 |
95 |
120 |
14 |
17 |
4 |
8 |
11 |
9 |
500 |
20 |
32 |
139 |
137 |
20 |
18 |
9 |
2 |
13 |
12 |
1500 |
17 |
19 |
136 |
131 |
17 |
7 |
4 |
7 |
10 |
10 |
5000 |
14 |
19 |
103 |
138 |
16 |
25 |
7 |
8 |
12 |
13 |
Positive control |
170 |
553 |
426 |
604 |
424 |
70 |
389 |
68 |
52 |
87 |
Table 2: Number of revertants per plate (second experiment) (mean of 3 plates) |
||||||||||
|
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
WP2 uvrA |
|||||
Conc. [µg/plate] |
- MA |
+MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
DMSO |
24 |
30 |
119 |
134 |
28 |
15 |
8 |
11 |
14 |
16 |
50 |
18 |
29 |
110 |
137 |
24 |
11 |
6 |
6 |
11 |
13 |
150 |
25 |
29 |
118 |
131 |
32 |
16 |
6 |
5 |
14 |
14 |
500 |
22 |
29 |
113 |
122 |
23 |
14 |
6 |
6 |
14 |
18 |
1500 |
19 |
34 |
107 |
126 |
26 |
15 |
7 |
6 |
15 |
20 |
5000 |
21 |
29 |
113 |
121 |
30 |
18 |
6 |
7 |
16 |
18 |
Positive control |
147 |
458 |
628 |
572 |
478 |
78 |
497 |
53 |
96 |
181 |
Applicant's summary and conclusion
- Conclusions:
- The results of the Bacterial Reverse Mutation Assay indicate that, under the conditions of this study, Dimethyl disulphide did not cause a positive response in either the presence or absence of Aroclor-induced rat liver S9.
- Executive summary:
Dimethyl disulfide, was tested in the Bacterial Reverse Mutation Assay using Salmonella typhimurium tester strains TA98, TA100, TA1535 and TA1537 and Escherichia coli tester strain WP2 uvrA in the presence and absence of Aroclor-induced rat liver S9. The assay was performed using the plate incorporation method. The dose levels tested were 1.5, 5.0, 15, 50, 150, 500, 1500 and 5000 µg per plate in the initial toxicity-mutation assay and 50, 150, 500, 1500 and 5000 µg per plate in the confirmatory mutagenicity assay. No positive mutagenic response was observed. Neither precipitate nor appreciable toxicity was observed. Dimethyl disulfide was concluded to be negative in the Bacterial Reverse Mutation Assay
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.