Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-036-0 | CAS number: 603-35-0
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: comparable with guideline study with acceptable restrictions; partly limited documentation
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
- Principles of method if other than guideline:
- Comprehensive testing programme on organophosphorus pesticides.
- GLP compliance:
- no
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Triphenylphosphine
- EC Number:
- 210-036-0
- EC Name:
- Triphenylphosphine
- Cas Number:
- 603-35-0
- Molecular formula:
- C18H15P
- IUPAC Name:
- triphenylphosphine
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- - purity > 98%
Test animals
- Species:
- mouse
- Strain:
- other: series 615 (no further data)
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- corn oil
- Details on exposure:
- Groups of 10 mice (5 males and 5 females) were treated daily by intraperitoneal injection on four consecutive days with doses corresponding to 80, 40, 20 and 10% of the LD50 (4 day-values; test substance in corn oil). 24 hours after the last treatment, the animals were sacrificed, and bone marrow cells were obtained from the sternum. Slides were produced using standard methods. 1000 polychromatic erythrocytes were scored per animal for the incidence of micronuclei. Cyclophosphamide (100 mg/kg bw, administered on two days) was used as positive control. Negative controls received corn oil (solvent).
- Duration of treatment / exposure:
- 4 days
- Frequency of treatment:
- daily
Doses / concentrationsopen allclose all
- Remarks:
- 80% of the LD50
- Remarks:
- 60% of the LD50
- Remarks:
- 40% of the LD50
- Remarks:
- 20% of the LD50
- Remarks:
- 10% of the LD50
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide (100 mg/kg bw, administered on 2 days)
Examinations
- Tissues and cell types examined:
- Bone marrow
- Details of tissue and slide preparation:
- Slides were produced using standard methods
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
- Executive summary:
The test substance did not induce micronuclei in bone marrow cells of mice. No details given on results of treated animals, negative and positive controls. However, 6 out of the 21 substances tested in this in vivo micronucleus assay yielded positive results, indicating that the test system was functional.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
