Tricalcium bis(orthophosphate)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study, data originate in part from a secondary source

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control and 100 mg/L. Samples were taken after 1, 24, 48, 72, and 96 hours.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 518 mg of the test substance was directly dissolved in 5,000 mL of dilution water and homogenized to prepare a test solution of 100 mg/L. The control used dilution water only.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Japanese rice fish
- Source: The Ecotoxicology Research Team, Korea Institute of Toxicology (KIT), KRICT, Deajeon, Korea (origin: Laboratory of Freshwater Fish stocks, Bioscience Center, Nagoya University, Japan)
- Age at study initiation (mean and range, SD): 3-4 month
- Length at study initiation (length definition, mean, range and SD): 2.8 cm (S.D. = 0.1 cm)
- Weight at study initiation (mean and range, SD): 0.18 g (S.D. = 0.02g)
- Feeding during test: none


ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: same as in test
- Feeding frequency: not fed 24 hours prior to test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

41.7 mg/L as CaCO3

Test temperature:

23.7 ± 0.2 °C

pH:

7.13 - 7.95

Dissolved oxygen:

5.38 - 8.3 mg/L

Nominal and measured concentrations:

Nominal test substance concentration: 100 mg/L
The concentrations of the test substance were measured to be 0.53-2.14 mg/L at 100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Aeration:none
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Filtered tap water (1 µm, membrane filter), residual chlorine or organic substances were removed by filtering through an activated carbon filter.
- Chlorine: < 0.01 mg/L


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and abnormality after 1, 24, 48, 72, and 96 hours
- Light intensity: 780-800 lux

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: no mortality observed at 100 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 2.14 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 2.14 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

There were no mortalities and adverse effects observed in any of the fish exposed to control and 100 mg/L treatment groups during the test. All results are expressed in terms of nominal concentration as the concept of loading rate.

Reported statistics and error estimates:

No statistics performed

Description of key information

Tricalcium bis(orthophosphate) is not acutely toxic to fish.

Additional information

The acute toxicity of tricalcium bis(orthophosphate) to fish was investigated in a study following OECD guideline 203 (Kim et al. 2013). The test was set up as a limit test under static conditions. Fish of the species Oryzias latipes were exposed to a nominal test substance concentration of 100 mg/L. The analytical recovery rate of the test substance was 0.53-2.14 mg/L at 100 mg/L nominal concentration. During the test period of 96 hours mortality and abnormal behavior of the test organisms were examined after 1, 24, 48, 72, and 96 hours. Mortality or adverse effects of the test substance were not observed. Based on the mean measured concentration a LC50(96 h) > 2.14 mg/L (nominal: LC50(96 h) >100 mg/L) was determined.

Based on the available data tricalcium bis(orthophosphate) is considered not acutely toxic to fish.