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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
- Water solubility (under test conditions): < 100 mg/L
Analytical monitoring:
yes
Details on sampling:
Sampies for the concentration analysis were taken at time points 0 (measurement vessel) and
48 hours (all test vessels) after start of exposure.
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Schering AG, Institute for Experimental Toxicology
- Age at study initiation (mean and range, SD): 0 - 24 hours
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
no
Test temperature:
19.5 - 20.7°C
pH:
7.9 - 8.5
Dissolved oxygen:
5.3 - 8.9 mg/L
Nominal and measured concentrations:
nominal: 0.9, 2.0, 4.5, 10, 22, 48 mg/L
mean measured: 1.0, 2.3, 4.4, 8.7, 17.2, and 37.0 mg/L
Details on test conditions:
20 Daphnia were used for each test solution and 20 tor the dilution water control, randomly distributed into groups of five. LabelIed 100 ml test vessels were filled with 45 ml of the test solution or tap water (contral group) and 5 mL of the dilution water with the introduction of the Daphnia. The test was performed with 4 replicates of each test solution and the dilution water contral with 5 Daphnia in each vessel. One additional test vessel for the test concentration and the control solution, respectively, was used without Daphnia for the measurements of pH, temperature and oxygen content.
The Daphnia were exposed to the test solutions and the dilution water for a period of 48 hours under static conditions. The light/dark rhythm was adjusted to 16 hours/8 hours.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
21.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 17.6-26.8
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
1 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
2.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Cumulative immobilization:

Concentration of ZK 5155 No. of Daphnia No. of immobilized Daphnia
(mg/L) 24 hours 48 hours
0 20 0 1
1.0 20 0 0
2.3 20 0 1
4.4 20 0 2
8.7 20 0 3
17.2 20 3 5
37.0 20 2 19
Reported statistics and error estimates:
The EC 50 was calculated with a computer programme based on probit analysis.

One immobilized Daphnia magna was observed in the control group after 48 hours. An immobilization of one individual (= 5%) is within the limit accepted by the OECD-Guideline for the control group. At 24 hours, only 10 -15% immobilized daphnids were observed at the two highest concentrations.

Validity criteria fulfilled:
yes
Remarks:
immobilisation and other abnormalities in the controls did not exceed 10 % by the end of the test; dissolved oxygen concentration remained above 3 mg/L throughout the exposure period
Conclusions:
After 48 h an EC-50 of 21.4 mg/L was measured for Androst-4-ene-3,17-dione towards Daphnia. The NOEC for 48 h was 1.0 mg/L, the LOEC was 2.3 mg/L. The results are relating to measured concentrations.
Executive summary:

Acute toxicity to Daphnia magna was determined in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). 20 Daphnia each were exposed to the different test concentrations of androstedione (1.0, 2.3, 4.4, 8.7, 17.2, and 37.0 mg/L; mean measured) over 48 hours. The pH, temperature and O2was 7.9-8.5, 19.9-20.7 C and 5.3-8.9 mg/L, respectively. The concentration of Androst-4-ene-3,17-dione was measured by HPLC/UV at 0 and 48 hours. The EC50 was calculated based on probit analysis.

The 48 h-EC50 was 21.4 mg/L (measured concentration). At 24 hours, only 10-15% immobilized daphnids were observed at the two highest concentrations. The NOEC for 48 h was 1.0 mg/L, the LOEC was 2.3 mg/L. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Daphnia study.

Description of key information

After 48 h an EC-50 of 21.4 mg/L was measured for Androst-4-ene-3,17-dione towards Daphnia. The NOEC for 48 h was 1.0 mg/L, the LOEC was 2.3 mg/L. The results are relating to measured concentrations.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
21.4 mg/L

Additional information