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Ecotoxicological information

Ecotoxicological Summary

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Administrative data

Hazard for aquatic organisms

Freshwater

Hazard assessment conclusion:
PNEC aqua (freshwater)
PNEC value:
0.1 mg/L
Assessment factor:
100
Extrapolation method:
assessment factor
PNEC freshwater (intermittent releases):
1 mg/L

Marine water

Hazard assessment conclusion:
PNEC aqua (marine water)
PNEC value:
0.01 mg/L
Assessment factor:
1 000
Extrapolation method:
assessment factor

STP

Hazard assessment conclusion:
PNEC STP
PNEC value:
10 mg/L
Assessment factor:
100
Extrapolation method:
assessment factor

Sediment (freshwater)

Hazard assessment conclusion:
no hazard identified

Sediment (marine water)

Hazard assessment conclusion:
no hazard identified

Hazard for air

Air

Hazard assessment conclusion:
no hazard identified

Hazard for terrestrial organisms

Soil

Hazard assessment conclusion:
no hazard identified

Hazard for predators

Secondary poisoning

Hazard assessment conclusion:
no potential for bioaccumulation

Additional information

Short-term toxicity to fish

The test item, Pigment Red 4, was tested for acute toxicity on fish Poecilia reticulata.

A static limit test with a dispersed test item partly dissolved by using ultrasonic device was conducted. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Result: 96 h - LC50 > 100.0 mg.L-1

 

Short-term toxicity to aquatic invertebrates

The test item, Pigment Red 4, was tested in acute immobilisation test on Daphnia magna. The static test was performed according to method No. L 49 “Acute Toxicity for Daphnia, immobilization test. Chemical formulations and waste waters”.

Result: EC50 (48h) > 100 mg·L-1

 

Toxicity to aquatic algae

The test item, Pigment Red 4, was tested for growth inhibition on algae Desmodesmus subspicatus.

The test was performed according to method C.3. - Freshwater Algae and Cyanobacteria, Growth Inhibition Test, Commission Regulation (EU) No.2016/266.

Result: 72 hour – ErC50 > 100 mg·L-1 (nominal concentration)

 

Toxicity to microorganisms

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out as a limit test for both Monoazo Red Pigments category members - C.I. Pigment Red 3 and C.I. Pigment5. The test was performed under static conditions with the nominal limit concentration of 1000 mg/L. The test item is not toxic at concentrations ≤ 1000 mg/L to activated sludge of a municipal sewage treatment plant.

  

Long-term toxicity to aquatic invertebrates

The test item, Pigment Red 4, was tested in reproduction test on Daphnia magna.

The test was performed according OECD Test Guideline No. 211 - Daphnia magna Reproduction Test. Adopted: October 2, 2012.

Result: 21 days – EC50 > 10 mg/L (nominal concentration)

 

Long-term toxicity to soil macroorganisms

The toxic effects of a Monoazo Red Pigments category member - C.I. Pigment Red 3 - on survival, growth, and reproduction of the earthworm Eisenia fetida were assessed during a test period of eight weeks based on the OECD Guidelines for Testing Chemicals, Test No. 222 (April 2004), the International Standards112682: 1998(E) and theGuidelineRichtlinien für die Prüfung von Pflanzenschutzmitteln im Zulassungsverfahren“ Part VI, 2-2, January 1994.

Mortality and reproduction was not affected and the NOEC was at least > 1000 mg/kg soil.

 

Toxicity to Terrestrial Plants

Seedling emergence and seedling growth in an OECD 208 study with C.I. Pigment Red 3 for 10 different plant species did not reveal any toxicity and NOEC was > 1000mg/kg soil. However, test with Beta Vulgaris is still ongoing due to inconclusive test results.

Conclusion on classification

As no toxicity for any tested species of algae, aquatic invertebrates, fish, microorganisms and soil organisms (Eisenida fetida) could be found neither in acute nor in chronic tests, no environmental classification is proposed.