Registration Dossier
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EC number: 226-866-1 | CAS number: 5521-31-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- It was not verified that the saturated dust concentration is the maximum possible.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 2,9-dimethylanthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-1,3,8,10(2H,9H)-tetrone
- EC Number:
- 226-866-1
- EC Name:
- 2,9-dimethylanthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-1,3,8,10(2H,9H)-tetrone
- Cas Number:
- 5521-31-3
- Molecular formula:
- C26H14N2O4
- IUPAC Name:
- 7,18-dimethyl-7,18-diazaheptacyclo[14.6.2.2²,⁵.0³,¹².0⁴,⁹.0¹³,²³.0²⁰,²⁴]hexacosa-1(22),2,4,9,11,13(23),14,16(24),20,25-decaene-6,8,17,19-tetrone
- Details on test material:
- Test materials used in this dossier are all considered to fall under the definition of nano-materials according to the European Commission Recommendation 2011/696/EU as the synthesis and manufacturing of this pigment always yields particulate material with a fine particle size distribution.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Bantin and Kingman Ltd., Grimston, Aldbrough, Nr. Hull
- Age at study initiation: 7-8 weeks
- Weight at study initiation: 190-370 g
- Housing: solid floor polypropylene cages furnished with sterilised sawdust, in groups of 5
- Diet (e.g. ad libitum): Rat and Mouse No. 1 Expanded Diet (BP Nutrition (UK) Ltd., Stepfield, Witham, Essex), ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: for a minimum of 3 days before exposure
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3
- Humidity (%): 50 ± 10
- Air changes (per hr): 8-10
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: cylindrical Perspex exposure chamber
- Exposure chamber volume: 7 L
- Rate of air: 15 L/min
- System of generating particulates/aerosols: Timbrell dust generator was used to produce the atmosphere
- Method of particle size determination: CS5 cascade impactor was employed with 6 separation stages covering the particle aerodynamic mass median diameter range 0.35-4 µm. Upper class limits for the 6 stages were 0.35, 0.5, 0.75, 1.0, 2.0 and 4.0 microns.
TEST ATMOSPHERE
- Brief description of analytical method used: The absolute concentration of the atmosphere generated was measured gravimetrically before and after each exposure period using a glass fibre filter disc placed adjacent to the outside wall of the exposure chamber.
- Samples taken from breathing zone: yes
TEST ATMOSPHERE (if not tabulated)
- MMAD (Mass median aerodynamic diameter): 0.87 µm - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- gravimetric determination
- Duration of exposure:
- 4 h
- Concentrations:
- 0.41 mg/L (analytical concentration)
- No. of animals per sex per dose:
- 10
- Control animals:
- yes
- Details on study design:
- The rats were observed for toxic or pharmacological effects during exposure and subsequently at least twice daily through a 14 day observation period. All observations were recorded daily. Body weights were determined on the day before exposure, immediately after exposure and on days 1, 3, 7, 10 and 14 after the day of exposure. Mortalities were recorded at daily intervals. Gross necropsy was performed at the end of the study.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 0.41 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: no mortality observed
- Mortality:
- No deaths occurred during exposure or during the observation period as a result of exposure to the test material. One male treated rat suffocated as a result of turning around in the restraining tube. A planned sacrifice was made of one female treated rat, one male control and one female control rat after exposure to assess the degree of primary lung irritation.
- Clinical signs:
- other: Treated rats had chromodacryorrhoea for a few hours after exposure and their pelts remained stained red throughout the observation period. Control rats had nasal secretion and chromodacryarrhaea on the day of exposure presumably an irritant effect of the
- Body weight:
- The mean body weights of the male and female control rats rose throughout the experiment, slowly until day 2 but thereafter more rapidly. The mean body weights of the treated rats were depressed following exposure but raised steadily throughout the observation period.
- Gross pathology:
- All the control rats and 6 male and 7 female treated rats, sacrificed after the 14 day observation period showed a moderate degree of pulmonary congestion and edema was present in some control rats. A similar degree of congestion was also noted in the female treated rat examined immediately after exposure, but congestion was more severe in the male, which had suffocated. No congestion was noted in the two control rats sacrificed at the same time, and no other abnormalities were noted in any of the control animals. The treated rats examined after exposure had red stained pelts.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the conditions chosen, the test substance was practically non-toxic.
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