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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment. Data from collection of data.

Data source

Reference
Reference Type:
publication
Title:
Acute Toxicities of Organic Chemicals to Fathead Minnows (Pimephalis promelas)
Author:
Geiger, D.L. et al.
Year:
1988
Bibliographic source:
Vol. 4, 189 - 190. Center for Lake Superior Environmental Studies, Univ. of Wisconsin, Superior, WI. (1988)

Materials and methods

Principles of method if other than guideline:
Method: according to U.S. EPA guideline, method similar to OECD guideline no. 203
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1,1,3,3-tetramethylbutylamine
EC Number:
203-491-1
EC Name:
1,1,3,3-tetramethylbutylamine
Cas Number:
107-45-9
Molecular formula:
C8H19N
IUPAC Name:
2,4,4-trimethylpentan-2-amine

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Sampling points were 0, 24, 48 and 72 h.

Test solutions

Details on test solutions:
Stock solution: 130 mg/L

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: Fathead minnow
- Source: US EPA Environmental Research Laboratory-Duluth or University of Wisconsin-Superior campus
- Age at study initiation: 29 days
- Length at study initiation: 19.7 +/- 2.0 mm
- Weight at study initiation (mean and range, SD): 0.119 +/- 0.037 g
- Feeding before test: frozen adult brine shrimp (Artemia sp.)
- Fish were not fed 24 h before and during the test

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
44.3 +/- 0.50 mg/L CaCO3
Test temperature:
24.3 +/- 0.26 °C
pH:
7.7 +/- 0.03
Dissolved oxygen:
7.5 +/- 0.27
Nominal and measured concentrations:
nominal concentrations: 0, 9.94, 15.3, 23.5, 36.1 and 55.6 mg/L
measured concentrations: <1.15, 9.22, 14.9, 24.2, 37.9 and 60.1 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
Tank volume: 1.0 L
- No. of organisms per concentration: 20
- Biomass loading rate: 2.380 g/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
24.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Basis for effect:
mortality (fish)
Remarks on result:
other: Conf. Lim. 22.1-27.2 mg/L
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
24.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Basis for effect:
behaviour
Remarks:
lost schooling behaviour, hyperactivity, overreactivity to external stimuli, increased respiration
Remarks on result:
other: Conf. Lim. 22.1-27.2 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
In this short-term fish toxicity test an LD50 value of 24.6 mg/L was obtained.