N-benzyl-N-C16-18 (even numbered)-alkyl-N-methyl-C16-18 (even numbered)-alkyl-1-aminium chloride

Short-term toxicity to aquatic invertebrates

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.66 mg/L

Additional information

The acute toxicity (inhibition of mobility) of N-benzyl-N-C16-18 (even numbered)-alkyl-N-methyl-C16-18 (even numbered)-alkyl-1-aminium chloride to Daphnia magna was assessed according to the method C2 of the European Directive 92/69/CEE and in the guideline 202 (part I) of the OECD. In order to avoid volatilisation of N-benzyl-N-C16-18 (even numbered)-alkyl-N-methyl-C16-18 (even numbered)-alkyl-1-aminium chloride, flasks were entirely filled with test solutions and closed with butyl rubber caps covered with PTFE. Daphnids were exposed in a static test to a concentration range of 0.207 to 1 mg/l. The test was performed with 5 daphnia per vessel. Testing flasks were incubated in darkness at 19 ± 0.5 °C. For each exposure concentration, the percentage of immobilisation after 24 hours and 48 hours was recorded. EC50-24h and EC50-48h were calculated with measured initial concentrations by regression analysis using the Probit/log model.

EC50 -48h = 0.66 mg/L

Concentrations of N-benzyl-N-C16-18 (even numbered)-alkyl-N-methyl-C16-18 (even numbered)-alkyl-1-aminium chloride were evaluated by measuring Dissolved Organic Carbon. The appearance of the test solutions was visually checked at the beginning and at the end of the test. Solutions were found to be clear,colourless, over the period of the test. One precipitation was observed at the end of the test. The study was performed in compliance with its quality criteria : immobilisation in the control did not exceed 10 % at the end of the test ; test daphnids in the control were not trapped at the surface of the water ; concentration of dissolved oxygen in the test vessels remained above 2 mg/l at the end of the test and pH did not vary by more than 1 unit.