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EC number: 201-175-8 | CAS number: 79-08-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August - October 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was performed according to the OECD guideline 202 and the laboratory was GLP certified.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Bromoacetic acid
- EC Number:
- 201-175-8
- EC Name:
- Bromoacetic acid
- Cas Number:
- 79-08-3
- Molecular formula:
- C2H3BrO2
- IUPAC Name:
- 2-bromoacetic acid
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material (as cited in study report): Monobromoacetic acid
- Substance type: hygroscopic white to yellow cristal
- Physical state: Solid
- Analytical purity: > 98%
- Purity test date: > 98%
- Lot/batch No.: 0271263
- Storage condition of test material: at room temperature - protected from light - freezer/refrigerator
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES are detailed in section 4 of the IUCLID dossier.
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- The concentrations of test substance in the test solutions were not determined by chemical analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was warmed to about 50°C and a quantity of 100 mg of monobromoacetic acid was accurately weighed out and dissolved in 100 0 ml of dilution water resulting in a solution containing 100 mg/l. From this solution 50, 90, 160 and 280 ml was taken and diluted to 500 ml with dilution water, resulting in test solutions of 10, 18, 32 and 56 mg/L.
- Controls: sythethic medium (DSWL) was used as a control medium.
The medium is prepared in large amounts (10,000 L) and the following components of the batch used for this test were checked by chemical analysis and found to be:
* Na+: 1.26 mmol.l-l
* K+: 0.23 mmol.l-1
* Ca2+: 1.41 mmol.l-1
* Mg2+: 0.78 mmol.I-l
* CI-: 2.81 mmol.l-1
* SO4 2-: 0.71 mmol.l-1
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation (mean and range, SD): < 24 h old
The organisms were cultured in the laboratory under standard conditions according to the principles of NPR 6503. Water-Necessaries, method and medium for the culture of Daphnia magna and the cultivation of the alga required as food. Nederlands Nonnalisatie-instituut, P.O. Box 5059, 2600 GB Delft, Netherlands (in Dutch).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- The hardness expressed as CaC03. was 210 mg.l-1.
The total organic carbon content was 2.0 mg.l-1. - Test temperature:
- 20 +/- 1°C
- pH:
- 6.7 - 8.2
- Dissolved oxygen:
- the lowest value: 7.3 mg/L
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal concentrations: 10, 18, 32, 56 and 100 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml all-glass beakers, each containing 100 ml of test solution or control medium.
- Aeration: not aerated during the test
- No. of organisms per vessel: 5 daphnia
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
TEST MEDIUM / WATER PARAMETERS
cf. Details on test solution
OTHER TEST CONDITIONS
- Photoperiod: 16h light- 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The immobile animals were counted after 24 hours and at the end of the test
TEST CONCENTRATIONS
- Range finding study: A range finding study allowed to establish the range concentration of the final study. However, the result were not included in the final report. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 57 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 42 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Condition: swimming behaviour, colour or any other visual observable morphological or behavioural criterion.
- Details on results:
- - there were no immobile animals in the control media.
- Reported statistics and error estimates:
- The EC50 values and their confidence interval were calculated by means of a parametric model developed by Kooijman.
Note: The slope of the concentration-effect curve was 73. Because the slope of the the concentration-effect curve was very steep, confidence intervals for the EC50 values or slope could not be calculated.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48h) on the mobility of Daphnia magna of bromoacetic acid is 42 mg/l, the NOEC (48h) is 32 mg/l. As bromoacetic acid is not bioaccumulating, has a log P<3.0 and is readily biodegradable no classification of bromoacetic acid for the environment is required based on this result.
- Executive summary:
The acute toxicity of monobromoacetic acid to the fresh-water crustacean Daphnia magna was detennined as described in the OECD Guideline no. 202 and according to the OECD principles of Good Laboratory Practice.
The test was carried out with 4 x 5 daphnias for each concentration, and the test solutions were not replaced.
The exposure duration was 48 hours.
The nominal concentrations tested were 10, 18, 32,56 and 100 mg/L.
The test substance appeared to be completely dissolved at all concentrations tested (visually assessed).
The results of the test were (in nominal concentrations):
* 24h EC50 (mobility)= 57 mg/l
* 48h EC50 (mobility)= 42 mg/l
Slope of the concentration-effect curve : 73
* 48h ECl00 (mobility)= 56 mg/l
* 48h NOEC (mobility = 48h ECO)= 32 mg/l
* 48h NOEC (condition)= 18 mg/l
The concentrations in this report refer to the test substance as supplied by sponsor.
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