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EC number: 201-175-8 | CAS number: 79-08-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August to October 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (OECD, EPA, etc)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Bromoacetic acid
- EC Number:
- 201-175-8
- EC Name:
- Bromoacetic acid
- Cas Number:
- 79-08-3
- Molecular formula:
- C2H3BrO2
- IUPAC Name:
- 2-bromoacetic acid
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material (as cited in study report): monobromoacetic acid
- Substance type: hygroscopic white to yellow crystals
- Physical state: Solid
- Analytical purity: > 98%
- Purity test date: > 98%
- Lot/batch No.: 0271763
- Storage condition of test material: stored at room temperature
- Other:
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES are detailed in section 4 of the IUCLID dossier.
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Not performed
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was warmed to about 50°C and a quantity of 2000 mg of monobromoacetic acid was accurately weighed out and dissolved in 2000 ml of dilution water (DSWL).
- Controls: DSWL
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DSWL
-Composition of the DSWL:
The nominal composition:
* Na+ 1.19 mmol/l
* K+ 0.20 mmol/l
* Ca2+ 1.36 mmol/l
* Mg2+ 0.73 mmol/l
* CI- 2.72 mmol/l
* SO42- 0.73 mmol/l
* HCO3- 1.39 mmol/l
This medium is prepared by addition of several salts to groundwater from a locality near Linschoten (the Netherlands). The groundwater contains several other trace elements (<< 1 mg/l).
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Brachydanio rerio
- Source: Commercial hatchery M.B. Ruysbrock B.V. (Noordvliet 159. Maassluis).
- Length at study initiation (length definition, mean, range and SD): 1.9 ± 0.1 cm
- Weight at study initiation (mean and range, SD): 0.05 ± 0.01 g
- Feeding during test: Not fed
ACCLIMATION: Not reported in the report
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Hardness expressed as CaCO3: 210 mg/L
The total organic carbon content: 2.0 mg/L - Test temperature:
- Varied between 24.6 and 25.2 °C
- pH:
- Varied between 3.6 and 8.1
Substance induced pH reduction:
* Nominal conc of test substance 0 mg/l (0h) --> pH: 8.1 (pH value just after dosing)
* Nominal conc of test substance 18 mg/l (0h) --> pH: 7.5 (pH value just after dosing)
* Nominal conc of test substance 32 mg/l (0h) --> pH: 7.3 (pH value just after dosing)
* Nominal conc of test substance 56 mg/l (0h) --> pH: 6.9 (pH value just after dosing)
* Nominal conc of test substance 100 mg/l (0h) --> pH: 6.5 (pH value just after dosing)
* Nominal conc of test substance 180 mg/l (0h) --> pH: 5.7 (pH value just after dosing)
* Nominal conc of test substance 320 mg/l (0h) --> pH: 3.6 (pH value just after dosing)
* Nominal conc of test substance 0 mg/l (96h) --> pH: 8.1 (pH value just before replacement)
* Nominal conc of test substance 18 mg/l (96h) --> pH: 8.1 (pH value just before replacement)
* Nominal conc of test substance 32 mg/l (96h) --> pH: 8.0 (pH value just before replacement)
* Nominal conc of test substance 56 mg/l (96h) --> pH: 8.0 (pH value just before replacement)
* Nominal conc of test substance 100 mg/l (96h) --> pH: 7.9 (pH value just before replacement)
Note: It is more likely that the pH influenced mortality at a concentration of 320 mg/l. Furthermore, it cannot be excluded that the pH value also affected the results at 180 mg/l. - Dissolved oxygen:
- Varied between 7.5 and 8.3 mg/l
- Nominal and measured concentrations:
- Initial test concentrations: 18, 32, 56, 100, 180 and 320 mg/l
Measured concentrations: not detremined - Details on test conditions:
- TEST SYSTEM
- Test vessel: 2000 ml all-glass beakers - 1500 ml of test solution
- Aeration: Slightly aerated
- Renewal rate of test solution (frequency/flow rate): Test solutions and control were replaced daily (semi-static).
- No. of organisms per vessel: 10 fishes
- No. of vessels per concentration: No replicates
TEST MEDIUM / WATER PARAMETERS
Dilution water (DSWL) - refer to "details on test solution" for further information on DSWL.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light-8h dark regime
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The dead animals were counted and removed at least after 24 h, 48 h, 72 h and 96 h.
At the same times the condition (swinuning behaviour, colour, respiratory function or any other observable morphological or visual behavioural criterion) of the survivors was visually compared with that of the control animals.
TEST CONCENTRATIONS
- Range finding study: yes to be able to determine final test concentration but no data/result reported in the study report.
- Test concentrations: 18, 32, 56, 100, 180 and 320 mg/l - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 103 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 180 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Remark for the NOEC value presented:
One animal was dead at 100 mg/l, but because 10% mortality (in the control) is allowed according to OECD 203, this concentration is recorded as the 96h NOEC, with regard to mortality as criterion.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to the test performed on Danio rerio, the substance monobromoacetic acid, is not considered as toxic. LC50(96h): 103 mg/l experssed in nominal concentration. Therefore, no classification of bromoacetic for the environment acid is required based on this test.
- Executive summary:
The acute toxicity of monobromoacetic acid to the fresh-water fish species Brachydanio rerio was determined as described in the OECD Guideline no. 203 and according to the principles of Good Laboratory Practice.
The test was carried out as a semi-static test with daily replacement of the test solutions and with 10 fishes for each concentration.
The exposure duration was 96 hours. The nominal concentrations tested were 18, 32, 56, 100 and 320 mg/l.
The test substance appeared to be completely dissolved at all concentrations tested (visually assessed).
The results of the test were (in nominal concentrations):
* 48h LC50 (mortality) 134 mg/l
* 96h LC50 (mortality) 103 mg/l
Slope of the concentration-effect curve 67
* 96h LCl00 (mortality) 180 mg/l
* 96h NOEC (mortality = 96h LC0) 100 mg/l
* 96h NOEC (condition) 100 mg/l
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